We narrowed to 7,632 results for: ALP

Showing: 6221 - 6240 of 7632 results

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pGG-TurboID-GFP-PTS1

Plasmid

#209406

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID-PTS1

Tags

GFP

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-GTSE1-His6

Plasmid

#69109

Purpose

For bacterial expression of recombinant human GTSE1 tagged with MBP and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

G2- and S-phase Expressed 1 (GTSE1 Human)

Tags

His6 and MBP

Expression

Bacterial

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD7 Flag

Plasmid

#74899

Purpose

Express COMMD7 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD7 (COMMD7 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Casp8

Plasmid

#48172

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of CASP8 (CASP8 Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pLVE-FH-IN

Plasmid

#70071

Purpose

2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-MBP-DmBam_13-36_AG

Plasmid

#148839

Purpose

Mammalian Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-POLArISact

Plasmid

#164970

Purpose

T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Use

In vitro transcription

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneoLuc-PP1A

Plasmid

#128348

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP1A (PPP1CA Human)

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Clover-Tubulin-C-18

Plasmid

#56374

Purpose

Localization: Microtubules, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDONR221 NLRP1 S1213A

Plasmid

#166824

Purpose

Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

S1213A

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD6 Flag

Plasmid

#74898

Purpose

Express COMMD6 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD6 (COMMD6 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-CAGGS-mNAGA

Plasmid

#196448

Purpose

Expresses modified α-N-acetylgalactosaminidase in mammalian cells

Depositor

Yuichiro Miyaoka

Article

Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897

Insert

modified alpha-N-acetylgalactosaminidase (NAGA Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB321

Plasmid

#68649

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFETCh_GATAD2A

Plasmid

#86279

Purpose

Donor vector for 3' FLAG tag of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GATAD2A homology arms (GATAD2A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP 5'CMV Δtat

Plasmid

#101347

Purpose

tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta Tat

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-AMV-α1L9'S-nAchR

Plasmid

#20684

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrna1 Mouse)

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Available Since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pSicoR-Ef1a-mCh-GFPi

Plasmid

#31849

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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HSF1 KO

Plasmid

#200207

Purpose

gRNA for HSF1 KO

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HSF1 KO (Hsf1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2E1

Plasmid

#67089

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2E1 (GTF2E1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only