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We narrowed to 10,841 results for: esp

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Showing: 6221 - 6240 of 10841 results

  1. pBItetDMPKSGFP

    Plasmid
    #96903
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Expression
    Mammalian
    Promoter
    tetracycline responsive and bidirectional
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96903-plasmid-map-sequence-id-495754

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  2. pSG424 ELKC(S383A)

    Plasmid
    #89334
    Purpose
    Elk1 cDNA encoding aa 307-428, aa383 mutated from ser-Ala, The PCR product was cut with BamH1 and Xba 1(365bp). pSG424 was cut with Bam H1 and Xba1. The Elk1 fragment was ligated into the BamH1
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.
    Insert
    ELK1 (ELK1 Human)
    Tags
    Gal 4
    Expression
    Mammalian
    Mutation
    encodes aa 307-428, changed serine 383 to alanine…
    Available Since
    April 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    89334-plasmid-map-sequence-id-176433

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  7. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    51260-plasmid-map-sequence-id-77871

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  12. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Michael Lynch
    Article
    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021
    Insert
    Cas9HF1
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    89961-plasmid-map-sequence-id-181339

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  15. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid
    #194328
    Purpose
    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
    Depositor
    Alexander McLellan
    Article
    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Tet-responsive promotor related mutations: G72V i…
    Promoter
    Tight tet-responsive promotor
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194328-plasmid-map-sequence-id-383152

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  16. pCGN-ATF6 (1-373)m1

    Plasmid
    #27174
    Purpose
    For mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.
    Depositor
    Ron Prywes
    Article
    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2
    Insert
    ATF6 (ATF6 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Contains only aa 1-373 Amino acids 315–317 are c…
    Available Since
    Jan. 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  17. pDual-eGFP(H66)

    Plasmid
    #63558
    Purpose
    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(H66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6221 - 6240 of 10841 results