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NK621

Plasmid

#196405

Purpose

Strong expression of firefly luciferase codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5actin-firefly-Sro3actin

Use

Luciferase

Mutation

N/A (synthetic)

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_69.11

Plasmid

#194214

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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XLone-mARG2

Plasmid

#173792

Purpose

Mammalian acoustic reporter gene 1 cassette 2 on XLone plasmid

Depositor

Xiaojun Lian

Article

Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

Insert

GvpN, F G L S K J U

Tags

GFP

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-miR21mutant-SunTag-TREAT

Plasmid

#196933

Purpose

SunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-let7-SunTag-TREAT

Plasmid

#196926

Purpose

SunTag cassette was added in the coding region in the let7-IRE-TREAT reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-let7R-SunTag-TREAT

Plasmid

#196927

Purpose

SunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0068

Plasmid

#196179

Purpose

YFP expression cassette: CaMV35S::TMV::YFP::35sT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

CaMV35S::TMV::YFP::35sT

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW1KN0351

Plasmid

#196173

Purpose

AtTGA1 expression cassette: CaMV35S::TMV::AtTGA1::35sT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

CaMV35S::TMV::AtTGA1::35sT

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSLS.601

Plasmid

#184955

Purpose

Express -Eco1 ncRNA library variants

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 ncRNA (variants)

Expression

Bacterial

Mutation

Library members with mutations described in manus…

Promoter

T7/lac

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK45 pHomL-RPL18Bp-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179029

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK44 pHomL-TEF2p-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179028

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-Isce-Tol2-Tn5-neo

Plasmid

#176619

Purpose

for generating PCR templates with Tol2 repeats and Isce sites for BAC recombination

Depositor

Nathan Lawson

Article

Shih et al Development. 2021 Nov 9. pii: 273393. do

Insert

I-SceI sites, Tol2 repeats and Tn5-neo cassette

Use

Unspecified

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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EC0_6_sgRNA

Plasmid

#163717

Purpose

Plasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

sgRNA expression cassette

Use

Synthetic Biology; Moclo vector level 0 (type 234)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-CT-Tagging

Plasmid

#165871

Purpose

Template for amplification of EGFP C-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - CT Tagging

Use

Synthetic Biology

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-CT-Tagging

Plasmid

#165869

Purpose

Template for amplification of mcherry C-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - CT Tagging

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-NT-Tagging

Plasmid

#165868

Purpose

Template for amplification of EGFP N-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb

Plasmid

#141067

Purpose

Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Synthetic Biology

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-NT-Tagging

Plasmid

#165870

Purpose

Template for amplification of mCherry N-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pIntegrase-AntiDsRed

Plasmid

#139677

Purpose

Cassette for testing phiC31 Integrase activity using a DsRed2 fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Integrase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDK038

Plasmid

#134182

Purpose

Expression of C. elegans KNL1

Depositor

Iain Cheeseman

Article

Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

Insert

KNL1 aa 69-235 (knl-1 Nematode)

Expression

Bacterial

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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