We narrowed to 23,594 results for: promoter

1. px330-GAPDH

   Plasmid

   #136940

   Purpose

   NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

   Depositor

   Jeremy Stark

   Article

   Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

   Insert

   sgRNA targeting GAPDH (GAPDH Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMHE-mClover3-MAP4-MTBD

   Plasmid

   #171498

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   So et al Science. 2019 Jun 28;364(6447). pii: 364

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   mClover3

   Promoter

   T7

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL-CRISPR.SFFV.PAC

   Plasmid

   #57829

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV-EF1aGFP-W

   Plasmid

   #159291

   Purpose

   Lentiviral vector expressing GFP driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   human EF1a promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

   Plasmid

   #160140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(nucleoplasmin)-3xHA-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EPB104

   Plasmid

   #68369

   Purpose

   contains human U6 promoter

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Insert

   U6 promoter (RNU6-1 Human)
   

   Expression

   Bacterial

   Promoter

   U6

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CamKII-ArchT-GFP (PV2527)

   Plasmid

   #99039

   Purpose

   AAV-mediated expression of ArchT under the CamKII promoter

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   ArchT-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS18 (mANGPTL3-Strep)

   Plasmid

   #109111

   Purpose

   Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

   Insert

   ANGPTL3 (Angptl3 Mouse)
   

   Tags

   StrepII

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. Accessory plasmid 2

   Plasmid

   #128571

   Purpose

   for cloning identified individual SPECS

   Depositor

   Timothy Lu

   Article

   Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagRFP

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP18 (CK2alpha' K69M)

    Plasmid

    #27090

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha' K69M (CSNK2A2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K69M

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pATP416-­ptetO7-­pphlO6-­crtYBI

    Plasmid

    #165976

    Purpose

    Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pphlO6), Synthetic promoter (…

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330_ATP1A1_G7_Dual_sgRNA

    Plasmid

    #173206

    Purpose

    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Dual U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-FLAG-GPD1

    Plasmid

    #32487

    Depositor

    Pere Puigserver

    Article

    Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

    Insert

    GPD1 (Gpd1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid

    #176458

    Purpose

    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

    Plasmid

    #139649

    Purpose

    Reporter assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    H2B-mCherry
    

    Use

    Lentiviral

    Tags

    FLAG, His6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDK4-luciferase full length (2135)

    Plasmid

    #86656

    Purpose

    Luciferase reporter containing CDK4 promoter

    Depositor

    Jaime Modiano

    Article

    Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

    Insert

    CDK4 promoter (CDK4 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    2120 base pairs including up to coding nucleotide…

    Promoter

    CDK4

    Available Since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF073 MBP-Axin-His

    Plasmid

    #154011

    Purpose

    Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAB07 (CK2beta)

    Plasmid

    #27091

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

19. ATG9A-2STrep

    Plasmid

    #210359

    Purpose

    Expresses ATG9A fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-T7-hOsa1

    Plasmid

    #17986

    Depositor

    Naoko Tanese

    Article

    Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

    Insert

    Osa1 (ARID1A Human)
    

    Tags

    T7 epitope

    Expression

    Mammalian

    Mutation

    Missing 5' end

    Available Since

    Aug. 19, 2008

    Availability

    Academic Institutions and Nonprofits only