59,541 results

1. pINTO-NFH::hSNRNP70

   Plasmid

   #65926

   Purpose

   Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Two silent mutations

   Promoter

   CMV/TO and CMV/TetO2 (T-REx)

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2003

   Plasmid

   #142902

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXD3 (FOXD3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. R777-E139 Hs.NF1

   Plasmid

   #70423

   Purpose

   Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   NF1 (NF1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF0915

   Plasmid

   #143451

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NCOA2 (NCOA2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-vhhGFP4-hIgG1-Fc

   Plasmid

   #105576

   Purpose

   T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
   

   Use

   In vitro transcription

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-FGFR1

   Plasmid

   #116740

   Purpose

   Lentiviral expression of FGFR1

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FGFR1 (FGFR1 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0584

   Plasmid

   #142651

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SRF (SRF Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTriEx-RhoA-wt_mScarlet-i_SGFP2

   Plasmid

   #85071

   Purpose

   Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
   

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-KDEL_TNF-SBP-RpHLuorin2

   Plasmid

   #171722

   Purpose

   Synchronize trafficking of TNFa-RpHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   TNF (TNF Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and ratiometri…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221-SLCO2B1_STOP

    Plasmid

    #161032

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLCO2B1 (SLCO2B1 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMK322 (TOP2A-mAC (Hygro))

    Plasmid

    #140653

    Purpose

    TOP2A tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

    Plasmid

    #107407

    Purpose

    Lentiviral expression of gRNA resistant PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    sg resistant PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Mutation

    sgRNA resistant

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF3505

    Plasmid

    #144981

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF12 (KLF12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-NLS-actin

    Plasmid

    #118380

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Mutation

    NLS

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-CSNK2A1

    Plasmid

    #23678

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. CLYBL hOPM

    Plasmid

    #112499

    Purpose

    Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locus

    Depositor

    Bruce Conklin

    Insert

    OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX317-VRK1

    Plasmid

    #199643

    Purpose

    Expresses VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHL-avitag3

    Plasmid

    #99847

    Purpose

    mammalian expression with secretive signal and avi tag for biotinylation

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Tags

    Avi

    Expression

    Mammalian

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-N2-Frankenbody-HA

    Plasmid

    #175292

    Purpose

    Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-HA frankenbody fused with mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3puro-mGFP-FXR1-isoform a

    Plasmid

    #225447

    Purpose

    Express EGFP-fusion protein of FXR1 isoform a

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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