We narrowed to 17,134 results for: Por

1. pDT-sgRNA-XMAS-1x

   Plasmid

   #164413

   Purpose

   Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJFRC-nSyb-IVS-PhiC31

   Plasmid

   #133868

   Purpose

   Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   PhiC31 Recombinase (int )
   

   Expression

   Insect

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3/hArf5(WT)-HA

   Plasmid

   #79427

   Purpose

   Expresses C-terminally HA-tagged Arf5(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

   Insert

   Arf5 (ARF5 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO-OMOR-eYFP

   Plasmid

   #66847

   Purpose

   Expresses Opto-MOR in a Cre dependent manner

   Depositor

   Michael Bruchas

   Article

   Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

   Insert

   Opto Mu opioid receptor
   

   Use

   AAV and Cre/Lox

   Tags

   eYFP

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115213

   Purpose

   Expresses RanCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   RanCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBa.GFP-KIF13B

   Plasmid

   #134626

   Purpose

   Mammalian expression of mouse KIF13B 442-1843

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF13B (Kif13b Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Chicken Beta actin

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 10XSTAT92E–luciferase

   Plasmid

   #37393

   Depositor

   Norbert Perrimon

   Article

   Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

   Insert

   SOCS36E enhancer fragment (Socs36E Fly)
   

   Use

   Luciferase; Jak/stat reporter construct

   Expression

   Insect

   Promoter

   Hsp70

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

   Plasmid

   #177183

   Purpose

   plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   sgRNA targeting the PEAR-GFP plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-FNLSHiFiNG-P2A-Puro

   Plasmid

   #136903

   Purpose

   Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFiNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Mutation

   R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs_FLAG-NMNAT2

    Plasmid

    #133260

    Purpose

    Express FLAG-tagged NMNAT2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT2 (NMNAT2 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKanCMV-CX4.3-7aa-mRuby3

    Plasmid

    #74254

    Purpose

    Mammalian expression of C-terminal fusion of mRuby3 to CX43

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    Connexin 43
    

    Tags

    mRuby3

    Expression

    Mammalian

    Promoter

    CAG and CMV

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid

    #202571

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTW072M

    Plasmid

    #115960

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl2 (nsP2 Q739L)
    

    Use

    Synthetic Biology

    Mutation

    nsP2 Q739L

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEYFP-Sec24D

    Plasmid

    #66614

    Purpose

    YFP fusion of Sec24D

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24D (SEC24D Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

15. CMV-PKCa-mEGFP-N1

    Plasmid

    #112269

    Purpose

    IDOCKSalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    Protein Kinase C alpha (PRKCA Bovine)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKanCMV-mClover3-18aa-actin

    Plasmid

    #74259

    Purpose

    Mammalian expression of N-terminal fusion of mClover3 to actin

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    Actin
    

    Tags

    mClover3

    Expression

    Mammalian

    Promoter

    CAG and CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pConRef-2H12

    Plasmid

    #221182

    Purpose

    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBa.GFP-AP1/gamma1

    Plasmid

    #218801

    Purpose

    Mammalian expression of mouse Ap1 g1

    Depositor

    Marvin Bentley

    Article

    Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

    Insert

    Ap1 g1 (Ap1g1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Chicken Beta actin

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGREAT2

    Plasmid

    #170890

    Purpose

    GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    p35SoTMV

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKanCMV-mRuby3-18aa-actin

    Plasmid

    #74255

    Purpose

    Mammalian expression of N-terminal fusion of mRuby3 to actin

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    actin
    

    Tags

    mRuby3

    Expression

    Mammalian

    Promoter

    CAG and CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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