We narrowed to 28,932 results for: Tat

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BK beta1

Plasmid

#113565

Purpose

mouse BK beta1 subunit in mammalian expression vector

Depositor

Robert Brenner

Article

Wang et al J Gen Physiol. 2006 Dec;128(6):731-44. d

Insert

Kcnmb1 (Kcnmb1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSC5

Plasmid

#104779

Purpose

pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1K3_M13ps_LacZα_gIII

Plasmid

#190435

Purpose

M13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

M13 packaging signal
M13 gIII
LacZα

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A

Plasmid

#8823

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A (GZMA Human)

Tags

(His)6 and Enterokinase site

Expression

Bacterial

Mutation

it mature enzyme.

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pET30b_OmpAT7Sav

Plasmid

#138589

Purpose

T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

Depositor

Markus Jeschek

Article

Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

Insert

T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export

Use

Synthetic Biology

Tags

OmpA export signal and T7 tag

Expression

Bacterial

Promoter

PT7

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TetON-CD4

Plasmid

#217537

Purpose

AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptor

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

CD4 receptor (CD4 Human)

Use

AAV

Tags

HA epitope

Expression

Mammalian

Promoter

TetOperon (TetO)

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPHB4 Sense

Plasmid

#60638

Purpose

Expresses the human EPHB4 exon 11/12 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Ephrin type B receptor 4 (EPHB4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGH153_MS2-AIDΔ-Hygro

Plasmid

#85416

Purpose

lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AIDΔ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgGOT1

Plasmid

#72874

Purpose

The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgGOT1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pXSE901BG

Plasmid

#91714

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pICH-chl-roGFP2-ORP1

Plasmid

#191699

Purpose

Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

chl-roGFP2-ORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-H29A

Plasmid

#80602

Purpose

Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-H29A

Use

AAV

Expression

Mammalian

Mutation

His29 mutated to Ala

Promoter

CBA

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBabepuro-HA-GASC1

Plasmid

#24182

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pBI-GFP-Bub1-wt

Plasmid

#16624

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Insert

BUB1 (BUB1 Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pT7-TaGu-ENT-3xF-ORF

Plasmid

#238488

Purpose

For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

R1109->A

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTK5

Plasmid

#37391

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

ACTR1A (ACTR1A Human)

Tags

S-tag, TEV, and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pICSL90002

Plasmid

#68261

Purpose

Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6-26 (Arabidopsis thaliana)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-smURFP-P2A-GFP

Plasmid

#197151

Purpose

Expresses the protein of smURFP-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

smURFP-P2A-GFP

Use

AAV

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pUC118-FLIP-FlpE-Puro

Plasmid

#84539

Purpose

a mock vector for generating FLIP-FlpE targeting vectors

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

FLIP-FlpE - Puro

Use

Cre/Lox and Mouse Targeting

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmOrange

Plasmid

#37135

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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