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pUC-DEST-R1R2

Plasmid

#141056

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJB089

Plasmid

#98914

Purpose

Express Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Inserts

FtsZ
ZapA

Tags

Dronpa and pAmCherry1

Expression

Bacterial

Promoter

T5-lac

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTU2-a-RFP

Plasmid

#72958

Purpose

Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - a site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pGL4.23-SCP1-ccdB

Plasmid

#131628

Purpose

Modified STARR-seq plasmid to measure enhancer activity (SCP1 promoter; ccdb gene at cloning site)

Depositor

Kevin White

Article

Liu et al Genome Biol. 2017 Nov 20;18(1):219. doi:

Type

Empty backbone

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SCP1

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR-P5P4

Plasmid

#141064

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-USP44-HIS

Plasmid

#43894

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Tags

HIS

Expression

Mammalian

Available Since

April 12, 2013

Availability

Academic Institutions and Nonprofits only
pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

Plasmid

#183077

Purpose

Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and crRNA ending with a HDV ribozyme

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2-FLAG

Plasmid

#91944

Purpose

Bacterial expression of maize SAE1 and SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSBDEST.H

Plasmid

#79464

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDRFLIP42

Plasmid

#65518

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-Cit-attR1 and attR2-mCer-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP35

Plasmid

#65513

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-TFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP32

Plasmid

#65511

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJC225

Plasmid

#176883

Purpose

MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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MTK4a_020

Plasmid

#123833

Purpose

Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d4PEST tag

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_007

Plasmid

#123760

Purpose

Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PTS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0530

Plasmid

#128987

Purpose

Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-ZiF268DBD-CRY2PHR

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-V220A

Plasmid

#47645

Purpose

pAC-EsaR with a c659t mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-V220A

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Valine 220 to Alanine

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pFA-GFP

Plasmid

#133009

Purpose

pFA carrying a sfGFP gene

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

sfGFP

Tags

6xhis

Expression

Bacterial

Promoter

tetR

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G10

Plasmid

#67144

Purpose

Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and sfGFP

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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LoriT

Plasmid

#163691

Purpose

Modified L4440 containing now CamR and oriT for conjugation

Depositor

Baris Tursun

Article

Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

Insert

oriT

Expression

Bacterial

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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