We narrowed to 10,402 results for: plasmids 101

1. pscAAV-Tyr

   Plasmid

   #135616

   Purpose

   Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

   Depositor

   Lin He

   Article

   Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

   Insert

   EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_FLAG-G1 PylRS

   Plasmid

   #154763

   Purpose

   plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAGGS-Flex/5'USS-GFP

   Plasmid

   #197884

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

   Plasmid

   #127314

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKG139

   Plasmid

   #69755

   Purpose

   Retroviral expression of CENP-C

   Depositor

   Iain Cheeseman

   Article

   Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

   Insert

   CENP-C (CENPC Human)
   

   Use

   Retroviral

   Tags

   mCherry-TEV-S

   Expression

   Mammalian

   Mutation

   L341F, N570D relative to NP_001803.2 (numbering b…

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPC-NMYC-mTRF1

   Plasmid

   #64162

   Purpose

   Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

   Depositor

   Titia de Lange

   Article

   Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

   Insert

   mTRF1 (Terf1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

   Plasmid

   #174891

   Purpose

   amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1_miRFP703

   Plasmid

   #183759

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   miRFP703
   

   Tags

   Lck lipidation signal

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

   Plasmid

   #120269

   Purpose

   Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

   Depositor

   Edward Callaway

   Article

   Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

   Insert

   eSyn-DIO-TVA950:YFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TetO-FUW-PLAGL1

    Plasmid

    #61542

    Purpose

    Expresses PLAGL1 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    PLAGL1 (PLAGL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.2 mir-1-1 reporter hsa-mir-122

    Plasmid

    #46673

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-122 (MIR122 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127315

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

    Plasmid

    #127312

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti.puro.MCV.ER.RAZ.2

    Plasmid

    #114382

    Purpose

    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.

    Depositor

    Sudhir Krishna, Reety Arora

    Article

    Arora et al Microb Pathog. 2019 Dec;137:103778. doi:

    Insert

    MCV NCCR and tumor T antigen regions
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MCV NCCR (viral own promoter)

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

    Plasmid

    #120268

    Purpose

    Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

    Depositor

    Edward Callaway

    Article

    Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

    Insert

    EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-miniSOG-VAMP2-citrine

    Plasmid

    #50969

    Purpose

    AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    miniSOG-VAMP2-citrine (Vamp2 Arabdopsis thaliana, Mouse)
    

    Use

    AAV

    Tags

    citrine and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-ASAP2f

    Plasmid

    #183757

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    ASAP2f
    

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-FLAG-Lin28A-mCCHC

    Plasmid

    #51372

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    Lin28A (LIN28A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CCHC domain mutant (H147A, H169A)

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

19. PB-TAC-OKMS-Cas9

    Plasmid

    #120353

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAT-9208

    Plasmid

    #124221

    Purpose

    Cloning plasmid for a self-targeting gRNA library

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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