We narrowed to 14,011 results for: flag

1. pAd-Klf4

   Plasmid

   #19770

   Depositor

   Konrad Hochedlinger

   Article

   Stadtfeld et al Science. 2008 Nov 7;322(5903):945-9. doi

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Adenoviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-FNLSHiFiNG-P2A-Puro

   Plasmid

   #136903

   Purpose

   Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFiNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCF CREB M1

   Plasmid

   #22969

   Depositor

   Marc Montminy

   Article

   Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

   Insert

   CREB (Creb1 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed Serine 133 to Alanine

   Promoter

   Available Since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. SETMAR-Cas9

   Plasmid

   #186701

   Purpose

   Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   SETMAR-Cas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SETD2(SET)-Cas9

   Plasmid

   #186700

   Purpose

   Plasmid encoding SETD2-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   SETD2(SET)-Cas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LbdCas12a KRAB

   Plasmid

   #188503

   Purpose

   Expresses FLAG tagged LbdCas12a KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN1-Lck-GCaMP2

   Plasmid

   #24794

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

   Insert

   Lck-GCaMP2
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Plasmid contains N-terminal 26 amino acid membran…

   Promoter

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. P823_eSpCas9(1.1)-RecA

   Plasmid

   #87264

   Purpose

   Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLEX+mCyclin D1

   Plasmid

   #86793

   Purpose

   mammalian expression of cyclin D1

   Depositor

   Charles Sherr, Martine Roussel

   Article

   Matsushime et al Cell. 1991 May 17;65(4):701-13.

   Insert

   cyclin D1 (Ccnd1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. GreenT-EC - GPI

    Plasmid

    #193946

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Use

    Tags

    GPI-anchor and Igk_secretion - HA-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GAL4-BRD4

    Plasmid

    #21943

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag and GAL4-DNA-BD

    Expression

    Bacterial

    Mutation

    aa 1209-1362

    Promoter

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

12. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJM60-S6K1(wt) barcoded

    Plasmid

    #48801

    Purpose

    expresses wt S6K1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGATCC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pLJM60 mUlk1 (wt) barcoded

    Plasmid

    #48799

    Purpose

    expresses wt Ulk1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    ULK1 (Ulk1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGATCC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMSCV-WT-mAbl

    Plasmid

    #69027

    Purpose

    Retroviral vector encoding WT mouse c-Abl

    Depositor

    Jean Wang

    Article

    Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

    Insert

    mouse Abl-Type IV (wild type)
    

    Use

    Retroviral

    Tags

    FLAG tag inserted at Internal SalI site (2934 bp)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

16. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiMutB3GALT5catdead-blast

    Plasmid

    #220869

    Purpose

    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    encodes D156A and D243A mutations; also, nucleoti…

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. vas-Cas9.874Z

    Plasmid

    #112687

    Purpose

    Express hSpCas9 under vasa promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    vasa

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTomo-AurA

    Plasmid

    #26293

    Depositor

    Inder Verma

    Article

    Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

    Insert

    aurora kinase A (AURKA Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOZC-PALB2

    Plasmid

    #133552

    Purpose

    PALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.

    Depositor

    David Livingston

    Article

    Xia et al Mol Cell. 2006 Jun 23. 22(6):719-29.

    Insert

    PALB2 (PALB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Cas12j2

    Plasmid

    #188498

    Purpose

    Expresses FLAG tagged Cas12j2

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSCVhygro-F-G9adSET

    Plasmid

    #41722

    Purpose

    Retroviral expression of human G9a mutant in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9adset (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TAL2430

    Plasmid

    #36829

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HDAC2-TALEN_Left (Hdac2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pDM098

    Plasmid

    #216818

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold
    

    Use

    Tags

    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LNCX BMP-RII

    Plasmid

    #12641

    Depositor

    Rik Derynck

    Article

    Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

    Insert

    BMP-RII (BMPR2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pEntr-Mef2aflbio

    Plasmid

    #32970

    Depositor

    William Pu

    Article

    He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

    Insert

    Mef2a (MEF2A Human)
    

    Use

    Gateway

    Tags

    flag-bio

    Expression

    Mutation

    Promoter

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pCS2 hSmad1-MM

    Plasmid

    #22990

    Depositor

    Edward De Robertis

    Article

    Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

    Insert

    Smad1 (SMAD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S214A, S206A, S195A, S187A

    Promoter

    Available Since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

28. dCas12j2 KRAB

    Plasmid

    #188508

    Purpose

    Expresses FLAG tagged dCas12j2 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D394A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 1198 pAdTrack FKHR

    Plasmid

    #9036

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHR (FOXO1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 28, 2005

    Availability

    Academic Institutions and Nonprofits only

30. TAL-L1-1.3-Ctrl

    Plasmid

    #102355

    Purpose

    Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-1.3
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    FLAG and HA

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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