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Addgene

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We narrowed to 10,596 results for: cat.1

Showing: 1241 - 1280 of 10596 results

  2. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    99698-plasmid-map-sequence-id-194602

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  6. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid
    #194890
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    194890-plasmid-map-sequence-id-392583

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  7. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194889
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194889-plasmid-map-sequence-id-382866

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  8. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid
    #194891
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194891-plasmid-map-sequence-id-382865

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  12. pETDuet1_NIX(1-182aa)-THROMBIN-GST (E72A/L75A/D77A/E81A; deltaWIPI2)

    Plasmid
    #223753
    Purpose
    Expression of recombinant protein for purification
    Depositor
    Sascha Martens
    Article
    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.
    Insert
    NIX (BNIP3L) soluble part (BNIP3L Human)
    Use
    Tags
    Thrombin cleavage-GST
    Expression
    Bacterial
    Mutation
    expresses aa 1-182, with E72A/L75A/D77A/E81A; del…
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    223753-plasmid-map-sequence-id-448300

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  14. pCMV-MA-VN

    Plasmid
    #123283
    Purpose
    Mammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    Matrix
    Use
    Tags
    VN: N-terminus of split Venus (a.a. V1–A154) I152…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123283-plasmid-map-sequence-id-239665

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  25. K560GFP

    Plasmid
    #129761
    Purpose
    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129761-plasmid-map-sequence-id-422377

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  26. pCDH-EF1s

    Plasmid
    #72484
    Purpose
    Express gene of interest under truncated EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72484-plasmid-map-sequence-id-134994

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  33. IBMc460

    Plasmid
    #161940
    Purpose
    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PPhlF
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161940-plasmid-map-sequence-id-329041

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  34. pTT5-hSDC1-N20

    Plasmid
    #52355
    Purpose
    expresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of SDC1 aa2-21 (according to numbering i…
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1241 - 1280 of 10596 results