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CRAF:BRAF BiBiT Vector

Plasmid

#238810

Purpose

Express CRAF-LgBiT_BRAF-SmBiT Fusion Proteins in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

CRAF (RAF1 Human)

Tags

LgBiT and SmBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)

Plasmid

#238182

Purpose

Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF4 (KLF4 Human, Synthetic)

Use

Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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SUMO_LPETG_MT3

Plasmid

#200304

Purpose

Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

Insert

metallothionein 3 (MT3 Human)

Tags

Smt3

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_N_ELF3

Plasmid

#187740

Purpose

MAC-tagged gene expression of human ELF3

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELF3 (ELF3 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-PYL1-sfGFP-PML

Plasmid

#167011

Purpose

expression of PYL1-SfGFP-PML, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

PML (PML Human)

Use

Lentiviral

Tags

GFP-PML

Expression

Mammalian

Available Since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry-SEC61B

Plasmid

#207671

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61B

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

SEC61B (SEC61B Human)

Tags

MCP-mCherry

Expression

Mammalian

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-HA-ATXN3

Plasmid

#22538

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

ATXN3 (ATXN3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pN3-SP4FL

Plasmid

#107719

Purpose

Expression plasmid for full-length human Sp4

Depositor

Guntram Suske

Insert

Sp4 (SP4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-SorLA-v1

Plasmid

#154892

Purpose

Expresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

SORL1 (SORL1 Human)

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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mAzami-Green-Galectin

Plasmid

#102419

Purpose

Expresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.

Depositor

Geert van den Bogaart

Article

Dingjan et al Sci Rep. 2016 Feb 24;6:22064. doi: 10.10

Insert

LGALS3 (LGALS3 Human)

Tags

mAzami-green

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLAS3W-S100P

Plasmid

#125009

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

S100P (S100P Human)

Expression

Mammalian

Promoter

CAG

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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hUbc13

Plasmid

#51131

Purpose

human ubc13, E2 for synthesis k63 linked ubiquitin chain

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

ubc13 (UBE2N Human)

Tags

His6 and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pLV-SspB-HaloTag-TIAM1-DHPH

Plasmid

#176127

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Lentiviral

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-hIFITM2

Plasmid

#58398

Purpose

Expressed human IFITM2 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM2 (IFITM2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pET30-2-GPD1

Plasmid

#83911

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_PACLight1

Plasmid

#197864

Purpose

Mammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)

Depositor

Tommaso Patriarchi

Article

Cola et al Elife. 2024 Aug 15;13:RP96496. doi: 10.7

Insert

PACLight1

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mCherry-Jph3 WPRE

Plasmid

#236237

Purpose

AAV expression of human Junctophilin3 N-terminally tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

Junctophilin 3 (JPH3 Human)

Use

AAV

Tags

mCherry

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cdk5GFP

Plasmid

#1346

Depositor

Li-Huei Tsai

Insert

CDK5 (CDK5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

Plasmid

#235086

Purpose

GFP splicing reporter with CORO1B intron

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)

Use

AAV

Expression

Mammalian

Promoter

custom bidirectional

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 actin-3XNLS P2A mCherry

Plasmid

#58475

Purpose

Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Inserts

beta actin (ACTB Human)
3XNLS
P2A cleavage site

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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