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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,214 results for: his

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Showing: 6421 - 6440 of 23214 results

  4. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60621-plasmid-map-sequence-id-208224

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  5. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  12. pGM202T7

    Plasmid
    #69993
    Purpose
    E. coli/Streptomyces expression vector containing the T7-promoter
    Depositor
    Günther Muth
    Article
    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(
    Inserts
    aphII
    tsr
    rep
    sso
    T7 promoter
    dso
    His-Orf
    Tags
    N-terminal and C-terminal His tag encoding sequen…
    Expression
    Bacterial
    Available Since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69993-plasmid-map-sequence-id-129665

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  19. pTDpelB-C_sfYFPTwinStrep

    Plasmid
    #45944
    Purpose
    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Insert
    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    Tags
    PelB export sequence and Twin-Strep-tag
    Expression
    Bacterial
    Promoter
    lacIq/Ptrc
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45944-plasmid-map-sequence-id-64657

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Showing: 6421 - 6440 of 23214 results