We narrowed to 6,683 results for: sas

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pSL0721 (pDonor_L1-105)

Plasmid

#130645

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short L)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0376 (His-MBP-StrepII-TniQ)

Plasmid

#130641

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite-StrepII on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0715 (pDonor_R1-57)

Plasmid

#130644

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short R)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUASp-FMW-attB-AthHen1

Plasmid

#104957

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

pTrasposase promoter

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP1

Plasmid

#160084

Purpose

Bacterial expression of Tn5-APEX2 fusion protein, no linker

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP2

Plasmid

#160085

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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MSI1_RRM2_pGEX

Plasmid

#228799

Purpose

Recombinant expression of mouse MSI1 RRM2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 RRM2 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI2_2xRRM_pGEX

Plasmid

#228800

Purpose

Recombinant expression of MSI2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI2_RRM1_pGEX

Plasmid

#228801

Purpose

Recombinant expression of MSI2 RRM1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 RRM1 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI2_RRM2_pGEX

Plasmid

#228802

Purpose

Recombinant expression of MSI2 RRM2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 RRM2 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_2xRRM_pGEX_Flag_noSBP

Plasmid

#228796

Purpose

Recombinant expression of mouse MSI1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 (Msi1 Mouse)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_SWAP_2xRRM_pGEX

Plasmid

#228797

Purpose

Recombinant expression of mouse MSI1 Swap with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 RRM2, RRM1 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_RRM1_pGEX

Plasmid

#228798

Purpose

Recombinant expression of mouse MSI1 RRM1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 RRM1 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-miR30-shJAG1 #4

Plasmid

#171197

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #4 (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shJAG1 #4

Use

Retroviral

Expression

Mammalian

Promoter

U6

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FRB-Fis1 (no mt-mKeima)

Plasmid

#223795

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FRB-Fis1

Use

Lentiviral

Tags

FRB

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208869

Purpose

For mammalian expression of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

HA

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-GST-TEV-GFP-NAP1

Plasmid

#208870

Purpose

For expression in E. coli of GFP-NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

GFP and GST-TEV

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pOPO794

Plasmid

#195303

Purpose

pBAD322-MycaIDCas-ΔCas6-RGR-PaqCI, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked PaqCI entry site.

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

McCAST Cascade Δcas6 and ribozyme flanked entry site

Expression

Bacterial

Promoter

Arabinose inducible arapBAD

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMS22: pHelper(PmcCAST)_entry_ΔTniQ

Plasmid

#168155

Purpose

Inducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS5: pHelper(AvCAST)_entry_ΔTniQ

Plasmid

#168138

Purpose

Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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