We narrowed to 58,550 results for: tra

1. psgRNA-GST-EGFP-GPIDAF

   Plasmid

   #213719

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Promoter

   CMV

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. psgRNA-GST-EGFP-GPICEAM7

   Plasmid

   #213721

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCLV3Cas-LT2

   Plasmid

   #210550

   Purpose

   Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
   

   Expression

   Plant

   Mutation

   1bp insertion after AAVS1 target sequence that di…

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYea5.1_Notch1-L24_sfGFP

   Plasmid

   #207865

   Purpose

   Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
   

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-6P-1 IntS12

   Plasmid

   #210521

   Purpose

   Expresses GST-tagged Drosophila IntS12

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   IntS12 (IntS12 Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYea5.1_Notch1_sfGFP

   Plasmid

   #207801

   Purpose

   Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein
   

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Promoter

   TEF

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAGFLEX.(cyto).iATPSnFR2.A95K.HaloTag

   Plasmid

   #209656

   Purpose

   Expression of iATPSnFR2, medium affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95K.HaloTag
   

   Use

   AAV

   Mutation

   A95K

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Shim_CytC_TP_GFP_pK7FWG2

   Plasmid

   #202646

   Purpose

   Determine where transit peptide targets GFP in N. benthamiana

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from putative Cytochrome C
   

   Tags

   Green Florescent Protien

   Expression

   Plant

   Available Since

   Aug. 10, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. HSF2 KO

   Plasmid

   #200208

   Purpose

   gRNA for HSF2 KO

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   HSF2 KO (Hsf2 Mouse)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1K245-->R GLUT1-GFP

    Plasmid

    #200095

    Purpose

    1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K245R

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TXNIP-py-FLAG

    Plasmid

    #200092

    Purpose

    TXNIP py motif mutant with C-terminal 3xFLAG tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    TXNIP (TXNIP Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    py motifs mutated (aa331-334 and 375-378)

    Promoter

    CMV

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBSKΔB-24xopto-TetO-no01

    Plasmid

    #174887

    Purpose

    Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    24xopto-TetO
    

    Use

    Southern blot probe

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut

    Plasmid

    #178910

    Purpose

    dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    3'UTR mMov10 (Mov10 Mouse)
    

    Use

    Luciferase

    Mutation

    CTG>AGT and TGC>CAT mir16+153 mutated

    Promoter

    SV40 promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLY237

    Plasmid

    #184150

    Purpose

    Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-R1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLY251

    Plasmid

    #184153

    Purpose

    Reprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-Ar2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLY283

    Plasmid

    #184156

    Purpose

    Reprogrammed short tracrRNA for hijacking mRNA of zntA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-zntA-1034
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCHECK2-mMOV10-3'UTR-MRE16-mut1

    Plasmid

    #178906

    Purpose

    dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    3'UTR mMov10 (Mov10 Mouse)
    

    Use

    Luciferase

    Mutation

    CTG>AGT miR16-5p MRE1 mutated

    Promoter

    SV40 promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. psiCHECK2-mMOV10-3'UTR-MRE153-mut4

    Plasmid

    #178909

    Purpose

    dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    3'UTR mMov10 (Mov10 Mouse)
    

    Use

    Luciferase

    Mutation

    TGC>CAT miR153-3p MRE mutated

    Promoter

    SV40 promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBO.ACT002

    Plasmid

    #182712

    Purpose

    aTc inducible dCasRx-IF1

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    3x(GGGS)-Escherichia Coli Initiation Factor 1

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPBO.ACT004

    Plasmid

    #182714

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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