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Showing: 6441 - 6460 of 40299 results

  3. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

    Plasmid
    #111389
    Purpose
    AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    ChETA-mRuby2
    W3SL
    Use
    AAV
    Tags
    mRuby2
    Expression
    Mammalian
    Mutation
    W3SL is built from a shortened WPRE, an upstream …
    Promoter
    hSynapsin
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111389-plasmid-map-sequence-id-212217

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  7. pJFA344C7

    Plasmid
    #49236
    Purpose
    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    TALE-TET1CD Backbone (TET1 Synthetic, Human)
    Use
    TALEN; Tale-tet1 expression vector
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    49236-plasmid-map-sequence-id-72802

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  9. Flag-M4-AR-S650D

    Plasmid
    #171246
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Ser650Asp (AR-S650D)
    Promoter
    CMV
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171246-plasmid-map-sequence-id-336097

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  10. Flag-M4-AR-S650A

    Plasmid
    #171245
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Ser650Ala (AR-S650A)
    Promoter
    CMV
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171245-plasmid-map-sequence-id-336092

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  11. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Available Since
    Oct. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159752-plasmid-map-sequence-id-313824

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  12. PA-DAD-wt

    Plasmid
    #53095
    Purpose
    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.
    Depositor
    Klaus Hahn, Ronen Zaidel-Bar
    Article
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Insert
    LOV(wt)-6aa-DAD
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus
    Available Since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    53095-plasmid-map-sequence-id-119520

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  14. pEGFP-NMHC-IIA-S1916A

    Plasmid
    #101040
    Purpose
    Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.
    Depositor
    Tom Egelhoff
    Article
    Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311
    Insert
    Non-muscle myosin IIA heavy chain (MYH9 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    S1916A, S1917A
    Promoter
    CMV
    Available Since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    101040-plasmid-map-sequence-id-198956

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  15. qTAG-C-mStayGold-Puro-CLTC

    Plasmid
    #227314
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227314-plasmid-map-sequence-id-451695

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  18. pJFA334E9

    Plasmid
    #49237
    Purpose
    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
    Use
    TALEN; Tale-tet1 expression vector
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    49237-plasmid-map-sequence-id-72803

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  19. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  20. qTAG-C-Solo-mStayGold-CLTC

    Plasmid
    #227313
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227313-plasmid-map-sequence-id-451694

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Showing: 6441 - 6460 of 40299 results