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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Plasmid

#187077

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in the mitochondria matrix

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Plasmid

#187078

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in lysosomes

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Tags

ZZ-3xTEV

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO761

Plasmid

#235760

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

3xFLAG

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO766

Plasmid

#235763

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

EGFP

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO933

Plasmid

#235765

Purpose

Expression of ScVPS34 delta C154-V202-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta C154-V202

Tags

3xFLAG

Expression

Yeast

Mutation

aa 154-202 deleted

Promoter

ScVPS34 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP

Plasmid

#233369

Purpose

CMV driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Use

Lentiviral

Tags

NES and mEGFP

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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E547* FL USP7

Plasmid

#229001

Purpose

E547-mutant of human USP7

Depositor

Irina Bezsonova

Insert

USP7 1-546 (USP7 Human)

Tags

6xHis cleavable with thrombin

Expression

Bacterial

Mutation

E547 mutated to stop codon

Promoter

T7

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pWR58

Plasmid

#202795

Purpose

integrating bioluminescene reporter for Cas9 activity in S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

P-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence

Expression

Yeast

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Pt NaV1.4

Plasmid

#200233

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Phyllobates terriblis NaV1.4 – muscle channel

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Dt NaV1.4

Plasmid

#200250

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Dendrobates tinctorius NaV1.4 – muscle channel

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SOD1

Plasmid

#222568

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-KDM5B

Plasmid

#222555

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of KDM5B

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

KDM5B (KDM5B Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-3xFLAG-SMAD1-active

Plasmid

#222579

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD1 (SMAD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

S487E, S489E

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pWR88

Plasmid

#203422

Purpose

empty BLINCAR plasmid for payload delivery, no payload, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

CAR copy 1
P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
CAR copy 2

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR23

Plasmid

#202770

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coKanMX
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR10

Plasmid

#202710

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coShBle
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR9

Plasmid

#202645

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR54

Plasmid

#203330

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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