We narrowed to 65,944 results for: des.1
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EMSV-myogenin (puro)
Plasmid#14712DepositorInsertmyogenin (Myog Rat)
ExpressionMammalianAvailable SinceApril 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
LRP6 deltaN-pCS2-VSVG
Plasmid#27281DepositorInsertlow density lipoprotein receptor-related protein 6 (LRP6 Human)
UseXenopus injectionTagsVSVGMutationcontains amino acid residues 1370–1613 (extracell…Available SinceJan. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Ub-D77
Plasmid#18896DepositorInsertUbiquitin-D77 (UBC Human)
ExpressionBacterialMutationAspartate residue is added to the C-terminus of t…Available SinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL1B
Plasmid#67066PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA3h
Plasmid#208703PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3h
UseAAVPromoterhSynAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3
Plasmid#189752PurposeProduces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet3
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163031PurposeFLiCRE TF with fast kinetics for neuronal expressionDepositorInsertNrxn3b-MKII-f-hLOV1-TEVcs-tTA
UseAAVExpressionMammalianPromoterSynapsinAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK1254
Plasmid#133057PurposeLentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.DepositorInsertTau.K18(LM)
UseLentiviralTagsmRuby2ExpressionMammalianPromoterEF1AAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon oG
Plasmid#131778PurposeINTRSECT 2.0DepositorInsertrabies glycoprotein - oG
UseAAV; IntrsectTagsnoneExpressionMammalianPromoterEf1aAvailable SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPFO*
Plasmid#120400PurposeExpresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.DepositorInsertMutant form of Perfringolysin O
TagsHis6 tagExpressionBacterialMutationAmino acids 1-27 are deleted, starts at amino aci…Available SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
sfTq2ox-PBP5
Plasmid#117960PurposePeriplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)DepositorInsertsfTq2ox-PBP5 (dacA )
ExpressionBacterialAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
PTP1B human
Plasmid#102719Purposehuman PTP1B catalytic domain (residues 1-301) in RP1B vectorDepositorInsertProtein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a dCas9-HA 2A Blast
Plasmid#102812PurposeLentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion proteinDepositorInsertdCas9-HA
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A (dCas9)PromoterEF1aAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE4497
Plasmid#80339Purposemammalian expression FnCpf1 nuclease and Fn crRNADepositorInsertsFn crRNA
FnCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
JT113-pETDuet1-(R)-hREV3L
Plasmid#64872Purposebacterial expression of human REV3LDepositorAvailable SinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP1594
Plasmid#65775PurposeHuman expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLSDepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
UseCRISPRTagsNLSExpressionMammalianPromoterCAGAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a]
Plasmid#49332PurposeRepaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
ExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…PromoterCMVAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgG2/λ
Plasmid#50367PurposeExpresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
ExpressionMammalianPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb2 SH2-SH3
Plasmid#46442DepositorInsertGrb2 (GRB2 Human)
TagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 1-217PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only
