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CC10-rtTA

Plasmid

#20179

Depositor

Jeffrey Whitsett

Article

Stripp et al J Biol Chem. 1992 Jul 25. 267(21):14703-

Insert

rcc10 promoter

Expression

Mammalian

Available Since

March 12, 2010

Availability

Academic Institutions and Nonprofits only
Double UP mNeon to mScarlet FRT

Plasmid

#141111

Purpose

Natively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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hOCT1-miRFP

Plasmid

#188995

Purpose

A mammalian expression plasmid encoding miRFP-tagged human OCT1

Depositor

Jeremy Baskin

Article

Chiu et al JACS Au. 2022 Nov 28;2(12):2703-2713. do

Insert

hOCT1 (SLC22A1 Human)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-talin1 rod

Plasmid

#32855

Depositor

Anna Huttenlocher

Article

Simonson et al J Immunol. 2006 Dec 1;177(11):7707-14.

Insert

Talin 1 Rod aa 434-2541 (Tln1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCM4.4

Plasmid

#217648

Purpose

an ermE*p-cas9-driven expression, as an alternative plasmid

Depositor

Huimin Zhao

Article

Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

Insert

Codon Optimized SpCas9

Use

CRISPR

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBbB6k-GFP

Plasmid

#35357

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt5a

Plasmid

#42279

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt5a (Wnt5a Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
p105_gRNA_Sa_CD40

Plasmid

#213781

Purpose

Expresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sa gRNA

Use

CRISPR and Lentiviral

Promoter

mU6

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-CC

Plasmid

#105116

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2/mVenus

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBbB8c-GFP

Plasmid

#35362

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-CN

Plasmid

#105119

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2 and mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJJS008

Plasmid

#188331

Purpose

PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::wrmScarlet::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMycMyt1-7zf/IRES-Red

Plasmid

#22652

Depositor

Lynn Hudson

Article

Nielsen et al Mol Cell Neurosci. 2004 Jan . 25(1):111-

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-mCherry

Plasmid

#32643

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
mCherry

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
DS-NMcas

Plasmid

#48646

Purpose

Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
NM tracrRNA

Use

CRISPR

Promoter

NM tracrRNA promoter and proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt7b

Plasmid

#42283

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt7b (Wnt7b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
mNP-Luc (PPARdelta promoter)

Plasmid

#16532

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR delta promoter NP (PPARD Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant binding sites

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pSLfa-PUb-EGFP-3'UTR#13

Plasmid

#176338

Purpose

The synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

Depositor

Zach Adelman

Article

Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

Insert

3'UTR#13

Expression

Insect

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-Sun

Plasmid

#119858

Purpose

Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-Sun

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-NN

Plasmid

#105125

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2/mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJJS005

Plasmid

#188328

Purpose

PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mNeonGreen::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJJS011

Plasmid

#188334

Purpose

PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mTagBFP2::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Lis1

Plasmid

#12574

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Lis1 (Pafah1b1 Mouse)

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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YIPlac204TKC-GFP-HDEL

Plasmid

#21771

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 2001 Apr 2. 153(1):47-62.

Insert

Green Fluorescent Protein (GFP)

Tags

HDEL and preKar2

Expression

Yeast

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry-C

Plasmid

#203326

Purpose

Express N-terminal mCherry-fused protein in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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FUG+p97W (C2)

Plasmid

#74018

Purpose

lentiviral expression of EGFP-SAP97beta fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

SAP97 beta isoform

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

hUbC

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pBbE8c-RFP

Plasmid

#35269

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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PKC delta dNPS

Plasmid

#16387

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta (Prkcd Mouse)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 15…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only