We narrowed to 13,906 results for: RING;

1. pALS3-Ma-SUMO-TAG35-sfGFP

   Plasmid

   #212120

   Purpose

   Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   SUMO-TAG35-sfGFP
   M. alvus Pyl-tRNA
   

   Use

   Tags

   His6 and SUMO-TAG35

   Expression

   Bacterial

   Mutation

   E35TAG (within SUMO encoding sequence)

   Promoter

   araC and lpp

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_D10A-1x7

   Plasmid

   #58777

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBTK1004 (pSL3)

   Plasmid

   #190999

   Purpose

   Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   red chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB045a

   Plasmid

   #79224

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ rpoZ-HA4; PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ; PlacIq

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_A0902

   Plasmid

   #91026

   Purpose

   Module A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TREX2-P2A-AtCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtUbi10

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG1.1-TSSK2/3xFLAG

   Plasmid

   #199625

   Purpose

   Expression vector of mouse TSSK2. 3xFLAG tag at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   Tssk2 mRNA (Tssk2 Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPTN1-209-EGFP

   Plasmid

   #68839

   Purpose

   deleted OPTN was inserted into pEGFP-N1 backbon

   Depositor

   Beatrice Yue

   Article

   Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-209 only

   Promoter

   CMV

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

8. BcLOV-mCh-EGFR

   Plasmid

   #208274

   Purpose

   Expresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

   Depositor

   Lukasz Bugaj

   Article

   Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   BcLOV-mCherry-EGFR
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   pCMV

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHiUGE V5-epitope donor ORF1

   Plasmid

   #200378

   Purpose

   Tagging endogenously expressed proteins with V5 epitope, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   V5-epitope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFC331

    Plasmid

    #87843

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    argB
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR_eGFP_191

    Plasmid

    #111821

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 171-191
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Bmp5

    Plasmid

    #163589

    Purpose

    Expresses BMP-5 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp5 (Bmp5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQ398X-EGFP

    Plasmid

    #68849

    Purpose

    mutated OPTN was inserted into pEGFP-N1 backbone

    Depositor

    Beatrice Yue

    Article

    Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q398X, results in truncation of the protein into …

    Promoter

    CMV

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAB035h

    Plasmid

    #79191

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Plac62 gIII, luxAB; Ptet rpoZ-HA4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac62; Ptet

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ290

    Plasmid

    #129670

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    AacCas12b is rice codon optimized

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBx1-VHH template1-3XMyc-Spacer

    Plasmid

    #176208

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template1-3XMyc-Spacer
    

    Use

    Tags

    Myc tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET FLIPE intramol-1u

    Plasmid

    #13549

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIP-E intramol WT (gltI E. coli)
    

    Use

    Tags

    EYFP

    Expression

    Bacterial

    Mutation

    ECFP insert in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETx-SR6c3

    Plasmid

    #176211

    Purpose

    Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    SR6c3
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pI2-MTU-DO-G418

    Plasmid

    #160018

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CrBlue ---- pcDNA3.1

    Plasmid

    #62917

    Purpose

    CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity.

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    CrBlue
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only