We narrowed to 14,478 results for: nts

1. pET22b-ecDHFR-dCys

   Plasmid

   #110541

   Purpose

   Bacterial protein of E.coli DHFR C85A/C152S mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   C85 mutated to A85, C152 mutated to S152

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. UBQ10:sXVE:ATG-3xHA-S11

   Plasmid

   #108238

   Purpose

   Binary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Insert

   ATG-3xHA-S11
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   UBQ10:sXVE:

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MDH1-PGK-FLAG-TAT-86-D

   Plasmid

   #35976

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   transactivating protein (tat Human Immunodeficiency Virus)
   

   Use

   RNAi and Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   From the "D" HIV clade

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MDH1-PGK-FLAG-TAT-86-C

   Plasmid

   #35982

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   transactivating protein (tat Human Immunodeficiency Virus)
   

   Use

   RNAi and Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   From the "C" HIV clade

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ127B

   Plasmid

   #99883

   Purpose

   Express two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Insert

   TALE-VP64-T2A-TALE-VP64
   

   Use

   TALEN

   Expression

   Plant

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTC130

   Plasmid

   #91178

   Purpose

   BeYDV T-DNA vector expressing TALENs targeting tomato ANT1, selectable marker gene removed from the backbone (template for amplification of the BaeI containing fragment to create pMOD_C0000)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALENs targeting tomato ANT1
   

   Use

   TALEN

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)

   Plasmid

   #91874

   Purpose

   Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Insert

   Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAtCas9-D10A_1

   Plasmid

   #91159

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A (nickase)
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTC366

   Plasmid

   #91215

   Purpose

   protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   2 gRNAs targeting tomato ARF8A
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_A0808

    Plasmid

    #91024

    Purpose

    Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Mutation

    D10A, H840A

    Promoter

    EC1.2

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_A0408

    Plasmid

    #91010

    Purpose

    Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Mutation

    D10A, H840A

    Promoter

    EC1.2

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD_A0708

    Plasmid

    #91021

    Purpose

    Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_H840A nickase
    

    Use

    CRISPR

    Mutation

    H840A

    Promoter

    EC1.2

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmcsg7-mCRT(D311A)

    Plasmid

    #83509

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Biol Chem. 2011 Mar 18;286(11):8771-85

    Insert

    CALR (Calr Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    D311A

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pmcsg7-mCRT(1-351)

    Plasmid

    #83508

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Biol Chem. 2011 Mar 18;286(11):8771-85

    Insert

    CALR (Calr Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    1-351 only

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pRSETa-Amrose v2

    Plasmid

    #79538

    Purpose

    pRSETa (Invitrogen) plasmid expressing Amrose variant 2

    Depositor

    Randolph Caldwell

    Article

    Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

    Insert

    Amrose v2
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGWB425

    Plasmid

    #74819

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT mir-1-1 reporter hsa-mir-205

    Plasmid

    #46744

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-205 (MIR205 Human)
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Promoter

    Metallothionein (MT)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT mir-1-1 reporter hsa-mir-128-1

    Plasmid

    #46743

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-128-1 (MIR128-1 Human)
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Promoter

    Metallothionein (MT)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.2 mir-1-1 reporter mir193b-wt

    Plasmid

    #46734

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir193b-wt (MIR193B Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.2 mir-1-1 reporter mir193b-3

    Plasmid

    #46733

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir193b-3 (MIR193B Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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