We narrowed to 11,688 results for: nts
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Plasmid#147118PurposeMammalian Expression of HsNxf-1-E151ADepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only
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pOPO636
Plasmid#195299PurposepACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).DepositorInsertTnsABCQ operon of McCAST (Tn7575)
ExpressionBacterialPromoterIPTG inducible lac promoterAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_msfGFP-SEPT7 Gmut2
Plasmid#180425Purposemammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFPDepositorAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1-14-GFP10
Plasmid#180343Purposemammalian expression of human SEPT9_i1 fused to GFP10DepositorAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-CAG-AAVS1
Plasmid#115262PurposeFor stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cellsDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Gmut
Plasmid#190479Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
ExpressionMammalianMutationc.1853A>GPromoterSV40Available SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal NLS
Plasmid#188398PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAM19A1-V5
Plasmid#184337PurposeExpression of V5-tagged mouse FAM19A1DepositorAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO - RBM28 E5 WT minigene
Plasmid#169269PurposeMammalian vector for constitutive expression of RBM28 Exon 5 WT minigeneDepositorAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-E484K-AVI
Plasmid#176444PurposeExpresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-P.1-AVI
Plasmid#176429PurposeExpresses SARS-CoV-2 NTD domain from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-P.1 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationL18F, T20N, P26S, D138Y, R190SAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mPpp3cc-FLAG-6XHIS
Plasmid#179160PurposeExpression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
Tags6XHIS and FLAGExpressionMammalianAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHelper_O
Plasmid#173783PurposeThe 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.DepositorInsert186 Integrase
UseSynthetic BiologyExpressionBacterialMutationE59G - please see depositor commentsAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode
Plasmid#170217PurposeBarcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)
Plasmid#160635PurposeTU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminatorDepositorInsertAcrIIA4
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
B-SpCas9
Plasmid#126760PurposeExpression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.DepositorInsertB-SpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationamino acids 1005-1013 replaced with two glycinePromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mTRAF3ip3-CFP
Plasmid#125181PurposeExpresses mouse TRAF3IP3 in mammalian cellsDepositorInsertTRAF3ip3 (Traf3ip3 Mouse)
TagsCFPExpressionMammalianMutationK87R, M283I, S359P, A506G- please see depositor c…PromoterCMVAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0113
Plasmid#117552PurposeLevel 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-J6LE60
Plasmid#103117PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertJ6LE60(Cas9 coding gene from Rhodovulum sp. PH10)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only