Addgene

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pET28a-T5-ASG_ClpXP

Plasmid

#153299

Purpose

Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

ASG

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-nTEVp

Plasmid

#179666

Purpose

Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Use

Tags

nTEVP

Expression

Mammalian

Mutation

K65E

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-cTEVp

Plasmid

#179667

Purpose

Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Use

Tags

cTEVP

Expression

Mammalian

Mutation

K65E

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMM0647

Plasmid

#129008

Purpose

Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorT(sp)

Plasmid

#111255

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorT signal peptide

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pc

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-2x

Plasmid

#124691

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTetR-BCD2-Ana GvpC

Plasmid

#153298

Purpose

Expresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquae

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Keratin 8 G61C

Plasmid

#18055

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Use

Tags

Expression

Mammalian

Mutation

G61C

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-4T1 14-3-3 epsilon K49E

Plasmid

#11945

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

K49E

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only
Keratin 8 S23A

Plasmid

#18057

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Use

Tags

Expression

Mammalian

Mutation

S23A

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pEF Itk Y511F myc

Plasmid

#20709

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk Y511F (Itk Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Y511F.

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pSCD-GSDMA-C

Plasmid

#226235

Purpose

Express GSDMA-C domain

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

Gasdermin A (Gsdma3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSCD-TVMVP

Plasmid

#226226

Purpose

Express TVMVP

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

TVMVP (TVMVgp1 Tobacco vein mottling virus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD_lambda_mRFP

Plasmid

#217831

Purpose

Yeast surface display Fab vector with lambda light chain and mRFP

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD_kappa_mRFP

Plasmid

#217830

Purpose

Yeast surface display Fab vector with kappa light chain and mRFP

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMMP_lambda

Plasmid

#217829

Purpose

Mini-mutagenesis plasmid for lambda light chain YSD Fabs

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMMP_kappa

Plasmid

#217828

Purpose

Mini-mutagenesis plasmid for kappa light chain YSD Fabs

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA337

Plasmid

#214750

Purpose

Medium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.7_Gag-dZF2-MCP-P2A-eGFP

Plasmid

#205523

Purpose

Express HIV Gag-delta-ZF2-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag delta ZF2-MCP

Use

Tags

Expression

Mammalian

Mutation

ZF2 deleted

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFP

Plasmid

#205529

Purpose

Express HIV GagZip-dp1dp6-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-dp1dp6-MCP

Use

Tags

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper, p1 and…

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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