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We narrowed to 24,925 results for: crispr

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Showing: 6581 - 6600 of 24925 results

  2. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  3. pEF:iCas9

    Plasmid
    #138267
    Purpose
    Expression of mTN3-GGS6-dCas9 (iCas9) in human.
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTN3-GGS6-dCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Cas9-D10A, H840A
    Promoter
    Ef1a
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138267-plasmid-map-sequence-id-273623

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  12. pX330.Pten.a

    Plasmid
    #66587
    Purpose
    sgRNA for Pten deletion
    Depositor
    Wen Xue
    Publication
    Li et al Genome Biol. 2015 May 28;16(1):111.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pten deletion (Pten Mouse)
    Expression
    Mammalian
    Available since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. AWP-031

    Plasmid
    #213967
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.
    Depositor
    Alexander Westermann
    Publication
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Synthetic, Segatella bryantii)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213967-plasmid-map-sequence-id-429166

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Showing: 6581 - 6600 of 24925 results