We narrowed to 146,921 results for: ins;

1. ACVR1-VSAS-R206H

   Plasmid

   #206185

   Purpose

   in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H

   Depositor

   Mary Mullins

   Article

   Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

   Insert

   activin A receptor type 1 (ACVR1 Human)
   

   Use

   In vitro transcription

   Tags

   FLAG tag

   Expression

   Mutation

   T189V, S192A, R206H

   Promoter

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ACVR1-VASS-R206H

   Plasmid

   #206186

   Purpose

   in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S190A, and R206H

   Depositor

   Mary Mullins

   Article

   Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

   Insert

   activin A receptor type 1 (ACVR1 Human)
   

   Use

   In vitro transcription

   Tags

   FLAG tag

   Expression

   Mutation

   T189V, S190A, R206H

   Promoter

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET32a-hEPGN

   Plasmid

   #199232

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Epithelial mitogen isoform 6 (EPGN Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF-G1TMSNKRS

   Plasmid

   #198323

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-MYO10-FERM-ITGBD

   Plasmid

   #194855

   Purpose

   Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

   Insert

   MYO10 S2001_F2002insA/T2009D (MYO10 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   MYO10 has the following mutations S2001_F2002insA…

   Promoter

   CMV

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G

   Plasmid

   #194462

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G

   Plasmid

   #194460

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Coexpressed VLP NES

   Plasmid

   #182433

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, GAL10, and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Coexpressed VLP NES (M)

   Plasmid

   #182435

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(M)
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, GAL10, and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Coexpressed VLP NES (G)

    Plasmid

    #182477

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, GAL10, and GAL7

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5-STABLE2-neo-RvCAHS3

    Plasmid

    #192472

    Purpose

    Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS3
    

    Use

    Tags

    T2A-EGFP-T2A-neoR

    Expression

    Insect

    Mutation

    Promoter

    Ac5 promoter

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Coexpressed Free PcPTS

    Plasmid

    #182483

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    PTS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Linked Free NES (AG4TGGA)2

    Plasmid

    #182488

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Linked Free (PT)4P

    Plasmid

    #182490

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SYKA-c022

    Plasmid

    #175490

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid

    #174646

    Purpose

    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    CMV (with TetOn)

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB80-KIF1A(1-365)-mVenus-SSPB(micro)

    Plasmid

    #174635

    Purpose

    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Use

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB80-KIF1A(1-383)-GFP-SSPB(micro)

    Plasmid

    #174627

    Purpose

    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTD73

    Plasmid

    #172897

    Purpose

    Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTD81

    Plasmid

    #172902

    Purpose

    Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge