We narrowed to 13,987 results for: flag

1. pSKA413

   Plasmid

   #80381

   Purpose

   Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

   Depositor

   Mustafa Khammash

   Article

   Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

   Inserts

   ccaS (complement)
   ccaR (complement)
   gfpmut3
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   FLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   ccaR and cpcG2Δ59

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1199 pAdTrack FKHR AAA

   Plasmid

   #9037

   Depositor

   William Sellers

   Article

   Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

   Insert

   FKHR AAA (FOXO1 Human)
   

   Use

   Adenoviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   T24A S256A S319A

   Promoter

   Available Since

   Sept. 28, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CAG-miniSOG

   Plasmid

   #193913

   Purpose

   Control plasmid that expresses non-targeted miniSOG in mammalian cells

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLEX-m-cyclin D1 -T286A mutant

   Plasmid

   #86792

   Purpose

   mammalian expression of cyclin D1 T286A mutant

   Depositor

   Charles Sherr, Martine Roussel

   Article

   Matsushime et al Cell. 1991 May 17;65(4):701-13.

   Insert

   cyclin D1 (Ccnd1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T286A

   Promoter

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pscALPS SIV-MND2 vpx

   Plasmid

   #115811

   Purpose

   Encodes codon optimized 3xFLAG-SIVMND2 Vpx (AY159322) and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVMND2 Vpx (AY159322)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T1-Claspin-FL-wt

   Plasmid

   #31621

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

   Insert

   Claspin (CLSPN Human)
   

   Use

   Tags

   Flag, GST, and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pFU-MVIIA-PE

   Plasmid

   #24144

   Depositor

   Ines Ibanez-Tallon

   Article

   Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

   Insert

   omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Conus magus, Aequorea victoria, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRetroX-TRE3G-SopE

   Plasmid

   #221327

   Purpose

   Dox-inducible expression of SopE in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   SopE
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   TRE3GV

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(-)_mtZFN(-)2G

   Plasmid

   #62799

   Purpose

   mtZFN2G architecture bearing (-) FokI domain

   Depositor

   Michal Minczuk

   Article

   Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

   Insert

   mtZFN(-)2G
   

   Use

   Tags

   Internal FLAG tag immediately after F1B mitochond…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-SMAD3

    Plasmid

    #79507

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ166-iSpyMac

    Plasmid

    #149553

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    iSpyMacCas9
    

    Use

    CRISPR; Gateway compatible zispymaccas9 entry clo…

    Tags

    3X FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    R221K, N394K, Protospacer interacting domain form…

    Promoter

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR8-Magneto2.0

    Plasmid

    #74333

    Purpose

    For gateway cloning Magneto2.0 into destination vector

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0
    

    Use

    Gateway vector

    Tags

    Flag

    Expression

    Mutation

    TRPV4: delta 760-871

    Promoter

    none

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pR008_APAD

    Plasmid

    #204818

    Purpose

    Expression Apobec1-ADAR2dd fusion protein as PIE-RBP control

    Depositor

    Xiaochang Zhang

    Article

    Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

    Insert

    rat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
    

    Use

    Tags

    HA and Flag and huADAR2dd

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. FKBP-CD28TMD-NTEVp(WT)

    Plasmid

    #137826

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(WT)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. N-BLa 1.1

    Plasmid

    #88994

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.1 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TALEN1297

    Plasmid

    #32279

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting hey2 (hey2 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMVTAG-NEMOL329P

    Plasmid

    #11971

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMOL329P (IKBKG Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Leucine329 to Proline

    Promoter

    Available Since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

18. plx208-CMV-Gal4DBD-CapN-SsrA-CapC

    Plasmid

    #213533

    Purpose

    Expresses one of the split transcription factor component in mammalian cells

    Depositor

    Wenjing Wang

    Article

    Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

    Insert

    SspB-EGFP-VP16
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TAL2261

    Plasmid

    #36700

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BMPR1A-TALEN_Right (BMPR1A Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

20. TAL2260

    Plasmid

    #36699

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BMPR1A-TALEN_Left (BMPR1A Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pCT

    Plasmid

    #60620

    Purpose

    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pESC-LEU-PmCDA1

    Plasmid

    #60818

    Purpose

    to overexpress lamprey deaminase in yeast

    Depositor

    Youri Pavlov

    Article

    Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

    Insert

    CDA1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. C-BLa 1.1

    Plasmid

    #88997

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    C-BLa 1.1 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. C-BLa 2.0

    Plasmid

    #92060

    Purpose

    The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.

    Depositor

    Dieter Langosch

    Article

    Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

    Insert

    C-BLa 2.0 fusion protein
    

    Use

    Tags

    Flag (DYKDHDG)

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFETCh_CIC_iso1

    Plasmid

    #135728

    Purpose

    Donor vector for 3' FLAG tag of human CIC_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    CIC_iso1 Homology arms (CIC Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFU-no toxin-PE

    Plasmid

    #24149

    Depositor

    Ines Ibanez-Tallon

    Article

    Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

    Insert

    PDGF-receptor-transmembrane domain (PDGFRB Aequorea victoria, Human)
    

    Use

    Lentiviral

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2010

    Availability

    Academic Institutions and Nonprofits only

27. LTB4R2B-Tango

    Plasmid

    #66424

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    LTB4R2B
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Promoter

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCLR2068

    Plasmid

    #28062

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains H537R point mutation in FokI domain, as …

    Promoter

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2 GFP-GSK3-MAPK-E3mut

    Plasmid

    #29691

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site mutated

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only