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FLAG-MED17

Plasmid

#15410

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED17 (MED17 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pJRoC30_Tv9nL

Plasmid

#188069

Purpose

PROSS-designed high-redox potential laccase from Trametes versicolor

Depositor

Sarel Fleishman

Article

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

PROSS-designed high-redox potential laccase from Trametes versicolor

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

79 PROSS mutations, described in publication

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR F87E)

Plasmid

#242846

Purpose

Bacterial expression of TTR

Depositor

Peter Wright

Article

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine, F8…

Promoter

T7

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR)

Plasmid

#238123

Purpose

bacterial expression of human TTR

Depositor

Peter Wright

Article

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine

Promoter

T7

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-IFT20

Plasmid

#218719

Purpose

Expresses C-terminally EGFP-tagged IFT20 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT20 (IFT20 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR

Plasmid

#182251

Purpose

Human PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene

Use

Luciferase

Expression

Mammalian

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-rab7 DN

Plasmid

#12660

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

T22N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1a S25N

Plasmid

#46777

Purpose

expression of S25N Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a S25N mutant (RAB1A Human)

Tags

myc

Expression

Mammalian

Mutation

S25N (dominant negative)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-LMP2A-IRES-mCherry

Plasmid

#220353

Purpose

Expresses EBV latent gene LMP2A in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p(A)n-PAUSE

Plasmid

#118068

Purpose

Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pKrox24(2xD-E)DsRed

Plasmid

#200111

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element, no other promoter elemen…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-eGFP

Plasmid

#116926

Purpose

Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

Depositor

Christophe Benoist, Diane Mathis

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

eGFP

Use

Retroviral

Promoter

EF1a

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B3-HA

Plasmid

#209211

Purpose

Mammalian expression of ATP8B3

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP8B3 (ATP8B3 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8s

Plasmid

#169256

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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amilCP_Pink chromoprotein

Plasmid

#117851

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP_Pink

Use

Synthetic Biology; Escherichia coli

Mutation

64A,65C, BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTH727-CEN-RLuc/staCFLuc

Plasmid

#38211

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pNUT N6His hTFNG

Plasmid

#67240

Purpose

Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

Depositor

Ross MacGillivray, Anne Mason

Article

Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

Insert

human serum transferrin (TF Human)

Tags

N-terminal signal peptide, 4 aa link, 6 His, Fact…

Expression

Mammalian

Mutation

Asn413 Asp, Asn611Asp

Promoter

SV40

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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scFLARE1-p2A-eGFP

Plasmid

#158699

Purpose

Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE1-p2A-eGFP

Use

AAV

Tags

Flag, V5. eGFP

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 EGFP-TEADi

Plasmid

#140145

Purpose

Tetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

EGFP TEADi

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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