19,658 results

1. pGEX-6P-1 Stu2 1-590

   Plasmid

   #38314

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-puro-Bcl-XL

   Plasmid

   #46972

   Purpose

   lentiviral expression of Bcl-XL

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-fDIO-Cre

   Plasmid

   #121675

   Purpose

   Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

   Depositor

   Esteban Engel, Alexander Nectow

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   NLS-CRE-HA
   

   Use

   AAV

   Tags

   3xHA and SV40-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-NuMA

   Plasmid

   #81029

   Purpose

   Expresses human NuMA with N-terminal EGFP tag in mammalian cells

   Depositor

   Michael Mancini

   Insert

   Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCP20T-tdTomato

   Plasmid

   #78469

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   tdTomato
   

   Expression

   Bacterial

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST14-DogCatcher

   Plasmid

   #171772

   Purpose

   Expresses DogCatcher protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mito-meGFP

   Plasmid

   #172481

   Purpose

   meGFP targeted to the mitochondrial matrix with a human COX8 presequence

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Tags

   meGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR - EGFP sgRNA 2

   Plasmid

   #51761

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EFS and U6

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJOE_pvdJ

   Plasmid

   #110142

   Purpose

   Suicide plasmid with repair template for knockout in P. putida

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-UBR5

    Plasmid

    #52050

    Purpose

    CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

    Depositor

    Darren Saunders

    Article

    Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

    Insert

    UBR5 (UBR5 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_221

    Plasmid

    #169142

    Purpose

    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

    Depositor

    John Doench, David Root

    Insert

    EGFP , EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-dCas9-5xPlat2AflD

    Plasmid

    #82560

    Purpose

    Expression vector for Cas9 peptide array (linker length: 22aa).

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD
    

    Expression

    Mammalian

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCBC-MT2T3

    Plasmid

    #50594

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    

    Use

    CRISPR; Pcr template

    Expression

    Plant

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lck-GFP

    Plasmid

    #61099

    Purpose

    Expression of a membrane-targeted form of eGFP

    Depositor

    Steven Green

    Article

    Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

    Insert

    Lck-GFP
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. mKeima-Red-N1

    Plasmid

    #54597

    Purpose

    Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mKeima-Red

    Expression

    Mammalian

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCherry-NLS

    Plasmid

    #39319

    Depositor

    Martin Offterdinger

    Article

    Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

    Insert

    mCherry-3xNLS
    

    Tags

    3xSV40LT NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. TOM20*-SBP-GFP

    Plasmid

    #120173

    Purpose

    Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
    

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Truncated TOM20: 1-30 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTagRFP657-N1

    Plasmid

    #31959

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Expression

    Mammalian

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUG6SP-tTA'

    Plasmid

    #41030

    Depositor

    Michael Nick Boddy

    Article

    Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

    Insert

    tTA' (tetR'-VP16)
    

    Available Since

    Dec. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST17-UbE2G1

    Plasmid

    #15790

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only