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pBabe RFP1-Smad2 neo

Plasmid

#58491

Purpose

mammalian expression of mRFP1-tagged mouse Smad2

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Smad2 (Smad2 Mouse)

Tags

mRFP1

Expression

Mammalian

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
pQCXIH-myc-hTAZ

Plasmid

#32841

Depositor

Kunliang Guan

Article

Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

Insert

TAZ (WWTR1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV/MSV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only
pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

Plasmid

#62956

Purpose

Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBA-mKeima-PIM

Plasmid

#174650

Purpose

Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

xXFKBP homo-mKeima-2x FKBP homo-2x FKBP

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

Chicken beta-actin

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-CCSS

Plasmid

#83424

Purpose

Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

Promoter

CMV

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHRi_1G4 hTCRβ-SNAP

Plasmid

#187604

Purpose

Lentiviral expression of hTCR beta(1G4), and SNAP for low expression

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

1G4 hTCR beta, and SNAP tag (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mHSP

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pT-GEM(0)

Plasmid

#62891

Purpose

Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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CAG-Cremyc-2A-GFP

Plasmid

#116879

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf GFP-vclb

Plasmid

#185411

Purpose

GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin b (vclb Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Human NANOS3-P2A-mCherry knock-in targeting construct

Plasmid

#59721

Purpose

Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

P2A mCherry

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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tol2-GFAP::CD14

Plasmid

#59593

Purpose

Magnetic cell sorting of ES cell-derived astrocytes

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

human CD14 (CD14 Human)

Expression

Mammalian

Promoter

gfa2

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-mAtg5

Plasmid

#38196

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf GFP-vcla

Plasmid

#185410

Purpose

GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin a (vcla Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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ETV4_pet28a

Plasmid

#131645

Purpose

Bacterial expression of ETS gene ETV4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV4 (ETV4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-NMHC-IIAdeltaIQ2

Plasmid

#35690

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP and GFPGenotRW

Expression

Mammalian

Mutation

The coding segment for aa 804-834 (corresponding …

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Tags

6xHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nrxn1beta AS4(-)-Fc

Plasmid

#59313

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)

Tags

Fc (from human IgG)

Expression

Mammalian

Promoter

CMV, T7

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183606

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Promoter

Mouse Pgk1

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee-Kras

Plasmid

#140838

Purpose

mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-Kras

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-PIPK(D253A)

Plasmid

#96811

Purpose

kinase-dead control for 5HT6-YFP-PIPK

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-PIPK(D253A)

Tags

YFP

Expression

Mammalian

Mutation

D253A inactivates kinase activity of PIPK

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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