59,544 results

1. pcDNA3.0-Magneto2.0-p2A-mCherry

   Plasmid

   #74308

   Purpose

   Bicistronic expression of Magneto2.0 and mCherry in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)
   

   Tags

   Flag and p2A-mCherry

   Expression

   Mammalian

   Mutation

   TRPV4: delta 760–871

   Promoter

   CMV

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-TRKC-V5/HIS

   Plasmid

   #205615

   Purpose

   Expression of human TRKC receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TRKC receptor tyrosine kinase (NTRK3 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. wt Opa1

   Plasmid

   #204427

   Purpose

   Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 protein.

   Depositor

   Keir Neuman

   Article

   Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

   Insert

   hOpa1 isoform 1 (OPA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV 3xFLAG-LC3A WT

   Plasmid

   #123089

   Purpose

   Expresses 3xFLAG-LC3A wild-type in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-HsProm1s1-mScarlet

   Plasmid

   #211330

   Purpose

   Mammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-hs-EGR1

   Plasmid

   #52724

   Purpose

   retroviral expression of human EGR1

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   EGR1 (EGR1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0219

   Plasmid

   #142563

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ADNP (ADNP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX2T E7

   Plasmid

   #13634

   Depositor

   Karl Munger

   Article

   Wu et al J Virol. 1993 Apr . 67(4):2402-7.

   Insert

   HPV 16 E7 (E7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pLKO.1-puro-cGASsh4

   Plasmid

   #127646

   Purpose

   Knock-down of human cGAS

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   cGAS shRNA (CGAS Human)
   

   Use

   Lentiviral

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. c-myc promoter (Del 2)

    Plasmid

    #16602

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 2) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-2 (KpnI->PvuII). The KpnI site is at -1194…

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

11. 3544 pMIG Bcl-2

    Plasmid

    #8793

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HA2-DDB1

    Plasmid

    #19909

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    Damage-specific DNA binding protein 1 (DDB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    please see depositor comment below

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. mH2A1.1-CT-GFP

    Plasmid

    #45167

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid

    #55201

    Purpose

    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-Flag-Rheb

    Plasmid

    #165031

    Purpose

    expression of Flag tagged Rheb protein in mammalian cells

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-HA-USP21

    Plasmid

    #22574

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP21 (USP21 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0266

    Plasmid

    #142578

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-Flag-CDK8-WT-IRES-mCherry

    Plasmid

    #68856

    Purpose

    Expresses Human CDK8 Wild-Type

    Depositor

    Matthew Shair

    Article

    Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCdc45-mCherry

    Plasmid

    #211380

    Purpose

    mammalian expression of human Cdc45 C-terminally fused to mCherry

    Depositor

    Helmut Pospiech

    Insert

    Cdc45 (CDC45 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Keratin 18 (pcDNA3)

    Plasmid

    #18064

    Depositor

    M Bishr Omary

    Article

    Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

    Insert

    Keratin 18 (KRT18 Human)
    

    Expression

    Mammalian

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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