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We narrowed to 14,439 results for: eng

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Showing: 6661 - 6680 of 14439 results

  1. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162685-plasmid-map-sequence-id-320886

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  4. pSCIP-hTRAC

    Plasmid
    #154094
    Purpose
    Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain
    Depositor
    Scott McComb
    Publication
    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA and BAIT targeting human TRAC locus
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154094-plasmid-map-sequence-id-307107

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  5. pcDNA3-P4:gs:nls:CIBN

    Plasmid
    #135986
    Purpose
    Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P4 coiled-coil fused to the CIBN domain
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135986-plasmid-map-sequence-id-269554

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  7. pHybr_r2a>mA-srt-his

    Plasmid
    #124806
    Purpose
    HDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Murine IgA
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124806-plasmid-map-sequence-id-244320

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  10. AAEL007584-Cas9

    Plasmid
    #100706
    Purpose
    The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Publication
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL007584-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Promoter
    AAEL007584 and Opie2
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100706-plasmid-map-sequence-id-193543

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  12. sgALK(B)

    Plasmid
    #85563
    Purpose
    Expresses sgRNA targeting human ALK around codon F1174
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  19. pcDNA3.1-KIT-D816V-V5/HIS

    Plasmid
    #236010
    Purpose
    expression of the D816V kinase defective mutant variant of human KIT that is associated with Acute Myeloid Leukemia and MASTSYS
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human KIT-D816V receptor tyrosine kinase, full length (KIT Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D816V substitution
    Promoter
    CMV
    Available since
    May 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    236010-plasmid-map-sequence-id-511408

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  20. TP1320

    Plasmid
    #252367
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Publication
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC nanobody E2
    Tags
    ALFA-sfGFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252367-plasmid-map-sequence-id-504519

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Showing: 6661 - 6680 of 14439 results