161,531 results

1. pTrcHIS-wt7D12

   Plasmid

   #125268

   Purpose

   Expresses wild type 7D12 anti-EGFR nanobodies

   Depositor

   Angus Johnston

   Article

   Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

   Insert

   7D12 nanobody
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Trc

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-ASH1L

   Plasmid

   #65369

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   ASH1L (ASH1L Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5 alpha6

   Plasmid

   #16036

   Depositor

   Filippo Giancotti

   Article

   Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

   Insert

   alpha6 (ITGA6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-HNRNPA1C-mCh-Cry2WT

   Plasmid

   #101226

   Purpose

   hnRNPA1(186-320) fused to mCh-Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   hnRNPA1(186-320) (HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF1095

   Plasmid

   #143923

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EN1 (EN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHCMV-VSVgSS-SNAP-TM

   Plasmid

   #207319

   Purpose

   Functional incorporation of SNAP-tag into envelope of virions

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SNAP-tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. eMagB-TgRFP-T

   Plasmid

   #162252

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagB-TgRFP-T
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-ATR Kinase dead

   Plasmid

   #31612

   Depositor

   Aziz Sancar

   Article

   Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K2327R (kinase dead)

   Promoter

   Available since

   Nov. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmScarlet-i_C1

   Plasmid

   #85044

   Purpose

   Mammalian expression of mScarlet-i

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   mScarlet-i
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Plasmid

    #163058

    Purpose

    roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    roGFP2-Orp1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. integrin beta6 EGFP-N3

    Plasmid

    #13593

    Depositor

    Dean Sheppard

    Insert

    integrin beta 6 (ITGB6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFPC1-huNFATc1EE-WT

    Plasmid

    #24219

    Depositor

    Jerry Crabtree

    Article

    Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Tags

    EE and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAd5-B5

    Plasmid

    #122555

    Purpose

    Block 5 for AdenoBuilder genome assembly

    Depositor

    Fred Bunz

    Article

    Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

    Insert

    Adenovirus 5 genomic region 17998-25062
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Use

    Tags

    Cerulean

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF0574

    Plasmid

    #143714

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXA13 (HOXA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. piggyBAC-H2B mCherry-XL (R125K)

    Plasmid

    #189926

    Purpose

    Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    CMV7

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. MEK1-GFP

    Plasmid

    #14746

    Depositor

    Rony Seger

    Article

    Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMP597

    Plasmid

    #134999

    Purpose

    Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV-CAG-K-SPOTIT

    Plasmid

    #191488

    Purpose

    Green fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    K-SPOTIT
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-HA2-ROC1

    Plasmid

    #19897

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMK293 (mAID-mCherry2-Hygro)

    Plasmid

    #72831

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Hygro
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE-TO-nmdCas9-3XmCherry

    Plasmid

    #64110

    Purpose

    Expression of Nm dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p5E-krt5

    Plasmid

    #82585

    Purpose

    5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression

    Depositor

    Kryn Stankunas

    Article

    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

    Insert

    zebrafish krt5 promoter (krt5 Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMCV-R17a

    Plasmid

    #24729

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    Full genome of Human Merkel Cell polyomavirus
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pORF1-Flag

    Plasmid

    #190565

    Purpose

    Mammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.

    Depositor

    Anthony Furano

    Article

    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    ORF1 (from L1.3)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA

    Plasmid

    #205442

    Purpose

    production of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminus

    Depositor

    Jeremy Kay

    Article

    Prigge et al Dev Cell. 2023 Oct 23;58(20):2080-2096.e

    Insert

    Unc5c (Unc5c Mouse)
    

    Use

    AAV

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    mini-CMV (with MVM small intron)

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAEL010097-Cas9

    Plasmid

    #100707

    Purpose

    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL010097 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCherry.90 alpha

    Plasmid

    #108222

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 alpha

    Depositor

    Didier Picard

    Article

    Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

    Insert

    mCherry-Hsp90a (HSP90AA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge