We narrowed to 1,337 results for: piggyBac

1. OA-986E

   Plasmid

   #125002

   Purpose

   Expresses dCas9-VP64 driven by the bottleneck promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   dCas9-VP64
   

   Use

   Piggyback

   Expression

   Insect

   Promoter

   bottleneck

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-NES-ZapCV2-Zinc dead

   Plasmid

   #203850

   Purpose

   Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

   Depositor

   Amy Palmer

   Article

   Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

   Insert

   ZapCV2-Ala zinc dead sensor
   

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   Cys and His in Zap1 is mutated with Ala

   Promoter

   CMV promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-NLS-ZapCV2-ALA

   Plasmid

   #203665

   Purpose

   Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

   Depositor

   Amy Palmer

   Article

   Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

   Insert

   ZapCV2-Ala zinc dead sensor
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Cys and His in Zap1 is mutated with Ala

   Promoter

   CMV promoter

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-scrCtnnd2.1-mCherry-CAAX

   Plasmid

   #209108

   Purpose

   Membrane tethered mCherry scramble of shRNA targeting Ctnnd2 N-terminus

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   scrCtnnd2.1
   

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-shCtnnd2.1-mCherry-CAAX

   Plasmid

   #209107

   Purpose

   Membrane tethered mCherry shRNA targeting Ctnnd2 N-terminus

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   shCtnnd2.1
   

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ePB-PURO-TT-PA-CRE

   Plasmid

   #179584

   Purpose

   ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

   Depositor

   Ali Hemmati Brivanlou

   Article

   De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

   Insert

   PA-MAG-CRE
   

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1010Av2

   Plasmid

   #125642

   Purpose

   Expresses FAR protein under 10xUAS control

   Depositor

   Omar Akbari

   Article

   Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

   Insert

   FAR protein
   

   Expression

   Insect

   Promoter

   10xUAS

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1010Bv2

   Plasmid

   #125643

   Purpose

   Expresses FAR protein under 10xUAS control

   Depositor

   Omar Akbari

   Article

   Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

   Insert

   FAR protein
   

   Expression

   Insect

   Promoter

   10xUAS

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-GG-OMKSL-PGK-Puro

   Plasmid

   #102894

   Purpose

   PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

    Plasmid

    #174610

    Purpose

    PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    human CD19, antigen minigene
    

    Expression

    Mammalian

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

    Plasmid

    #225350

    Purpose

    piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

    Depositor

    Wendell Lim

    Article

    Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

    Insert

    H2B (H2B Synthetic, Human)
    

    Tags

    miRFP and non-fluorescent GFP (Y67F)-P2A

    Expression

    Mammalian

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-short_BrokenHeart (no BC)

    Plasmid

    #86950

    Purpose

    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    Brokenheart construct
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-NES-ZapCV2

    Plasmid

    #222950

    Purpose

    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2 (NES Synthetic)
    

    Tags

    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid

    #102909

    Purpose

    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid

    #201254

    Purpose

    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

    Depositor

    Alexander Kleger

    Article

    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

    Insert

    KRAS (KRAS Human)
    

    Use

    Piggybac transposon

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    changed Gly (G) 12 to Asp (D)

    Promoter

    TRE

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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