We narrowed to 9,308 results for: try

1. pQE-WT-N4-R5-YRNRV

   Plasmid

   #63625

   Purpose

   N4-R5-YRNRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   N4-R5-YRNRV RNAP
   

   Use

   Tags

   Expression

   Mutation

   N671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …

   Promoter

   T5/Lac

   Available since

   June 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pBad-HPII TAG392

   Plasmid

   #105848

   Purpose

   expresses E. coli HPII catalase with TAG stop codon at site 392 and N-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   hydroperoxidase II catalase
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 392

   Promoter

   pBad/arabinose

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBad-HPII TAG415

   Plasmid

   #105847

   Purpose

   expresses E. coli HPII catalase with TAG stop codon at site 415 and N-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   hydroperoxidase II catalase
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 415

   Promoter

   pBad/arabinose

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBad-HPII TAG348

   Plasmid

   #105844

   Purpose

   expresses E. coli HPII catalase with TAG stop codon at site 348 and N-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   hydroperoxidase II catalase
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 348

   Promoter

   pBad/arabinose

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBad-HPII TAG283

   Plasmid

   #105843

   Purpose

   expresses E. coli HPII catalase with TAG stop codon at site 283 and N-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   hydroperoxidase II catalase
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 283

   Promoter

   pBad/arabinose

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYEXC3H

   Plasmid

   #49026

   Purpose

   FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYEXNHG3

   Plasmid

   #49025

   Purpose

   FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MBP-RGG-LgBit-RGG

   Plasmid

   #176643

   Purpose

   LgBit fused to LAF-1 RGG domain with N-terminal MBP tag

   Depositor

   Daniel Hammer

   Article

   Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

   Insert

   MBP-RGG-LgBit-RGG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Human Phosphomevalonate kinase (PMK)

   Plasmid

   #52430

   Purpose

   bacterial expression of human Phosphomevalonate kinase (PMK)

   Depositor

   Henry Miziorko

   Article

   Herdendorf et al Biochemistry. 2006 Mar 14;45(10):3235-42

   Insert

   Phosphomevalonate kinase (PMVK Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

10. SMX DEST vector

    Plasmid

    #62399

    Purpose

    Empty Backbone

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 8061-E43

    Plasmid

    #234280

    Purpose

    Gateway ORF Entry clone of mouse Bmpr1b with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    Bmpr1b (Bmpr1b Mouse)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 8522-E13

    Plasmid

    #234297

    Purpose

    Gateway ORF Entry clone of human SMAD3 with stop codon (for native or N-terminal fusions); T179V/S204A/S208A/S213A mutations (All four linker sites dead)

    Depositor

    Dominic Esposito

    Insert

    SMAD3 T179V/S204A/S208A/S213A (SMAD3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    T179V/S204A/S208A/S213A; All four linker sites de…

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 8522-E19

    Plasmid

    #234299

    Purpose

    Gateway ORF Entry clone of human TGFB3 with stop codon (for native or N-terminal fusions); C227S/C229S mutations

    Depositor

    Dominic Esposito

    Insert

    TGFB3 C227S/C229S (TGFB3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    C227S/C229S

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 9336-E23

    Plasmid

    #234306

    Purpose

    Gateway ORF Entry clone of human SMAD3 EPSM-CSD with stop codon (for native or N-terminal fusions); Linker sites dead, C-terminal constitutively active

    Depositor

    Dominic Esposito

    Insert

    SMAD3 EPSM-CSD (SMAD3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    T179V/S204A/S208A/S213A/S423D/S425D; Linker sites…

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 9336-E26

    Plasmid

    #234309

    Purpose

    Gateway ORF Entry clone of human SMAD3 LSD-CSD with stop codon (for native or N-terminal fusions); Linker and C-terminal sites all constitutively active

    Depositor

    Dominic Esposito

    Insert

    SMAD3 LSD-CSD (SMAD3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    T179D/S204D/S208D/S213D/S423D/S425D; Linker and C…

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEN_TT Linker

    Plasmid

    #192307

    Purpose

    Gateway entry vector for cloning

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    Linker
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SZ1-GST-mCherry-RGG

    Plasmid

    #176646

    Purpose

    SynZip1 linked surfactant protein that localizes to RGG condensate interface

    Depositor

    Daniel Hammer

    Article

    Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

    Insert

    SZ1-GST-mCherry-RGG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NK651

    Plasmid

    #109107

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Article

    Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

    Insert

    PTSG_07215 deltaCC
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Deletes C-terminal coiled-coil from PTSG_07215

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NK633

    Plasmid

    #109097

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Article

    Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

    Insert

    mTFP1_3'UTR Act
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NK617

    Plasmid

    #109095

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Article

    Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

    Insert

    S. cerevisiae CoxIV target sequence
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. NK624

    Plasmid

    #109094

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Article

    Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

    Insert

    HDEL peptide
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBad-HPII TAG206

    Plasmid

    #105846

    Purpose

    expresses E. coli HPII catalase with TAG stop codon at site 206 and N-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    hydroperoxidase II catalase
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 206

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBad-HPII TAG568

    Plasmid

    #105845

    Purpose

    expresses E. coli HPII catalase with TAG stop codon at site 568 and N-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    hydroperoxidase II catalase
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 568

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK392

    Plasmid

    #70236

    Purpose

    Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE-H40N

    Depositor

    T. J. Kappock

    Article

    Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    His40Asn

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNJB99

    Plasmid

    #51521

    Purpose

    Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Insert

    Zif268:FokI
    

    Use

    Gateway entry vector

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR4-FLAG (w210-2)

    Plasmid

    #17423

    Purpose

    Gateway entry vector for N-term FLAG tag

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMSCVpuro-Venus

    Plasmid

    #96940

    Purpose

    Retroviral plasmid containing Venus for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Venus Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-C1-6xHis

    Plasmid

    #87343

    Purpose

    To express EGFP-6xHis in mammalian cell

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    6xHis
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR4 no ccDB (686-1)

    Plasmid

    #17424

    Purpose

    Gateway entry vector, no ccDB gene

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME pHluorin2

    Plasmid

    #73794

    Purpose

    ratiometric pHluorin2 sequence in a middle entry clone

    Depositor

    David Raible

    Article

    Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

    Insert

    ratiometric pHluorin2
    

    Use

    Middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA TfR1-miniR

    Plasmid

    #181986

    Purpose

    MiniR genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniE labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    TfR1-MiniR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEZT-BM

    Plasmid

    #74099

    Purpose

    Multi-purpose rapid titer bacmam expression vector

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Bacmam, baculovirus

    Tags

    EGFP following p10 promoter

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, p10

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p-cyclinB1-mcherry (1958)

    Plasmid

    #26063

    Depositor

    Jonathon Pines

    Article

    Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENTR-LUC (w158-1)

    Plasmid

    #17473

    Purpose

    Gateway entry vector, Firefly Luciferase

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJK226

    Plasmid

    #71125

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENTR4-FKBP12DD N-term A (B3-1)

    Plasmid

    #17414

    Purpose

    Gateway entry vector for Degron, N-term, frame A

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    FKBP12DD (L106P)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Hs.NRAS G12D

    Plasmid

    #83176

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12D

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPB-CAG-rtTA-IN

    Plasmid

    #60612

    Purpose

    Backbone for tet-inducible genes

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR-D-Topo-hTRIM9

    Plasmid

    #51032

    Purpose

    entry vector for cloning human TRIM9

    Depositor

    Timothy Cox

    Article

    Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

    Insert

    TRIM9 (TRIM9 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1/hChR2-EYFP

    Plasmid

    #20939

    Purpose

    Mammalian expression of humanized ChR2 fused to EYFP

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge