We narrowed to 18,263 results for: Met;

1. hHS1-M7A,H102A

   Plasmid

   #159171

   Purpose

   Human codon-optimized cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   hHS1-M7A,H102A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated both Met 7 and His 102 of the cyt b562 mo…

   Promoter

   CMV

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBa-tdTomato-flag-BicD2 594-FKBP

   Plasmid

   #64205

   Purpose

   Bicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   Bicaudal D protein (Bicd2 Mouse)
   

   Use

   Tags

   FKBP, flag, and tdTomato

   Expression

   Mammalian

   Mutation

   no start methionine, aa1-594

   Promoter

   chicken Beta Actin

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX_305-N-dTAG_OGT

   Plasmid

   #155196

   Purpose

   N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x HA and FKBP12(F36V)

   Expression

   Mammalian

   Mutation

   lacking N-terminal Met

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBa-eGFP-flag-BicD2 594-FKBP

   Plasmid

   #63569

   Purpose

   Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   Bicaudal D protein (Bicd2 Mouse)
   

   Use

   Tags

   FKBP, eGFP, and flag

   Expression

   Mammalian

   Mutation

   no start methionine, aa1-594

   Promoter

   chicken Beta Actin

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVL1393-MAL-SYK

   Plasmid

   #157990

   Purpose

   Expresses SYK in insect cell (sf9)

   Depositor

   Byung Il Lee

   Article

   Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

   Insert

   Spleen tyrosine kinase (SYK Human)
   

   Use

   Tags

   Met-Ala-Leu and add Leu,Glu residues +His tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSicoR Dnmt1

   Plasmid

   #12166

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Insert

   Dnmt1 shRNA (Dnmt1 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   EGFP is expressed from this plasmid as a marker, …

   Promoter

   Available since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. cfmTurquoise-N

   Plasmid

   #139284

   Purpose

   Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

   Depositor

   Ikuo Wada

   Article

   Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

   Insert

   cfmTurquoise
   

   Use

   Tags

   Multi-cloning sites

   Expression

   Mammalian

   Mutation

   Cys48Ser and Cys70/Met

   Promoter

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSico Dnmt1

   Plasmid

   #12165

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Insert

   Dnmt1 shRNA (Dnmt1 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   EGFP is expressed from this plasmid as a marker, …

   Promoter

   Available since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET28-MRPP3-His

   Plasmid

   #67866

   Purpose

   bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

   Insert

   MRPP3 (KIAA0391 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino acid 46 of recombinant MRPP3 is preceded by…

   Promoter

   T7

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-MRPP3-myc-His

    Plasmid

    #67864

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mApple-M1 SpastinHFDD

    Plasmid

    #134462

    Purpose

    M1 SpastinHFDD expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met to Ala at amino acid 87; His to Asp at amino …

    Promoter

    CMV

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. nuc-yHS1-M7A,H102A

    Plasmid

    #159168

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1-M7A,H102A
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. mito-yHS1-M7A,H102A

    Plasmid

    #159165

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A,H102A
    

    Use

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. lentiCRISPR v2-sgDnmt1_1

    Plasmid

    #138347

    Purpose

    Express guide RNA 1 for Dnmt1

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    mouse Dnmt1 sgRNA (Dnmt1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR v2-sgDnmt1_2

    Plasmid

    #138348

    Purpose

    Express guide RNA 2 for mouse Dnmt1

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    mouse Dnmt1 sgRNA (Dnmt1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-STAG2-K225insNM

    Plasmid

    #31974

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    human STAG2 cDNA (STAG2 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    inserted amino acids Asn-Met before Lys225

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMBS-1125

    Plasmid

    #217999

    Purpose

    Start-methionine

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    Start-Met
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 289aa ELL2

    Plasmid

    #127266

    Purpose

    For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Three Mets (133, 138, 186) to Ileu. Contains 289…

    Promoter

    T7

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. frameshift ELL2

    Plasmid

    #127257

    Purpose

    For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    XhoI site between the 1st &2nd Met was filled…

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSIRV-NFAT-eGFP

    Plasmid

    #118031

    Purpose

    Fluorescent reporter for NFAT activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with NFAT binding site

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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