We narrowed to 54,283 results for: Ran;

1. ClhN-P1K-3VSV-Atg8-MET15

   Plasmid

   #207055

   Purpose

   Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   Promoter

   pATG8

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. P1K-3HA-Atg8∆R-MET15

   Plasmid

   #207034

   Purpose

   Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆R

   Promoter

   pATG8

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. P1K-3HA-Atg8∆GR-MET15

   Plasmid

   #207033

   Purpose

   Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆GR

   Promoter

   pATG8

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mTLR4

   Plasmid

   #13085

   Depositor

   Ruslan Medzhitov

   Insert

   TLR4 (Tlr4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZac2.1 U6-SaAi9L-U6-SaAi9R

   Plasmid

   #78604

   Purpose

   AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   gRNAs for SaCas9 targeting Ai9 locus
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42333

   Purpose

   This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

   Plasmid

   #207031

   Purpose

   Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZac2.1 SV40-CMV-SaCas9-3xNLS

   Plasmid

   #78601

   Purpose

   AAV vector containing SaCas9

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   SV40-CMV-SaCas9-3xNLS
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   SV40, CMV promoters

   Available since

   Dec. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pZac2.1 U6-SaDMDR7-U6-SaDMDL2

   Plasmid

   #78603

   Purpose

   AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   U6-SaDMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-EF1a-PGD2-1.1

    Plasmid

    #214764

    Purpose

    AAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.1 sensor
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF-1-alpha promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRII-Topo U6-SpAi9L-U6SpDMDL

    Plasmid

    #78608

    Purpose

    Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9L-U6SpDMDL
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pCRII-Topo U6-SpAi9R-U6-SpDMDR

    Plasmid

    #78609

    Purpose

    Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9R-U6-SpDMDR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pDisplay-PGD2-1.1-IRES-mcherry

    Plasmid

    #214763

    Purpose

    Express GRAB-PGD2-1.1 sensor and membrane tethered mCherry under CMV promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.1 sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP

    Plasmid

    #207025

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A I375A D384A Y387A

    Promoter

    pTEF1

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PCUP1-GFP-Pho8-406

    Plasmid

    #207006

    Purpose

    Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    PHO8
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PCUP1-GFP-Osh1-406

    Plasmid

    #207007

    Purpose

    Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    OSH1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDisplay-PGD2-1.0-IRES-mcherry

    Plasmid

    #214762

    Purpose

    Express GRAB-PGD2-1.0 sensor and membrane tethered mCherry under CMV promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.0 sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

    Plasmid

    #207050

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    P85L P86L

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

    Plasmid

    #207051

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    T56A R65A T87A

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

    Plasmid

    #207052

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    R65A P85A P86A

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge