We narrowed to 8,429 results for: BLI

1. AICSDP-57: SMC1A-mEGFP

   Plasmid

   #114406

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A

   Depositor

   Allen Institute for Cell Science

   Insert

   SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Sept. 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. Lenti_AID-Dam-lamin B1

   Plasmid

   #119764

   Purpose

   Lentiviral plasmid for Mammalian Expression

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   AID-Dam-lamin B1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-c5x_UnaG

   Plasmid

   #163125

   Purpose

   A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

   Depositor

   Julie Biteen

   Article

   Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

   Insert

   UnaG
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mApple-hCOL1A1

   Plasmid

   #140113

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2-CAGIG-LAMP1-mGL

   Plasmid

   #222394

   Purpose

   To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.

   Depositor

   Pantelis Tsoulfas

   Insert

   LAMP1 and mGreenlantern (LAMP1 Synthetic, Homo Sapiens, Human)
   

   Use

   AAV

   Tags

   LAMP1 and mGreenLantern

   Expression

   Mammalian

   Mutation

   Promoter

   CAG or CAGIG

   Available since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TO-hNGN2-BSD-mApple

   Plasmid

   #182308

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   hNGN2 (NEUROG2 Human)
   bsd-T2A-mycNLS-mApple
   

   Use

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. RabV CVS-N2c(deltaG)-dsRed

   Plasmid

   #73460

   Purpose

   Expresses dsRed for monosynaptic circuit mapping

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   dsRed
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1761 - pAAV SYN1 DIO HA-hM3D(Gq)

   Plasmid

   #125147

   Purpose

   An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM3D(Gq)) from a synapsin promoter

   Depositor

   Christopher Richie

   Insert

   HA-tagged hM3D(Gq) (CHRM3 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SYN1

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

    Plasmid

    #201186

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 1 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKOR1-mcs

    Plasmid

    #181755

    Purpose

    Allele replacement for Staphylococcus aureus

    Depositor

    Suzan Rooijakkers

    Article

    Stapels et al Proc Natl Acad Sci U S A. 2014 Sep 9;111

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

    Plasmid

    #191676

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A

    Depositor

    Veerle Baekelandt

    Inserts

    ATP10B (ATP10B Human)
    TMEM30A (TMEM30A Human)
    

    Use

    Lentiviral

    Tags

    6x HIS and flag

    Expression

    Mutation

    D433N

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ITCH

    Plasmid

    #36197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH (ITCH Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains HECT domain

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit HC

    Plasmid

    #221349

    Purpose

    Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit HC; to be used with Anti-Biotin [IPI-M33] light chain (Plasmid 221350) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Biotin [IPI-M33] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid

    #230058

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    Tags

    6X HIS, HRV 3C PreScission protease, HaloTag, and…

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid

    #230059

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 single chain variable fragment (scFv)
    

    Use

    Tags

    6X HIS, HRV 3C PreScission protease, HaloTag, and…

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDB179-HotPETase

    Plasmid

    #218701

    Purpose

    Encodes codon optimized HotPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    HotPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFP.R377

    Plasmid

    #138247

    Purpose

    Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AMPKA2

    Plasmid

    #36194

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    AMPKA2 (PRKAA2 Human)
    

    Use

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    contains KINASE DOMAIN only

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. RabV CVS-N2c(deltaG)-mCherry

    Plasmid

    #73464

    Purpose

    Expresses mCherry for monosynaptic circuit mapping

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    mCherry
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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