We narrowed to 10,749 results for: AGA

1. pFastBac-M13-6His-KIF1C-GFP

   Plasmid

   #130975

   Purpose

   Expression of 6His-KIF1C-GFP in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Use

   Tags

   6His and GFP

   Expression

   Insect

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   Polyhedrin

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMPUW-miR-124-GFP-Puro

   Plasmid

   #117321

   Purpose

   Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   hsa-miR-124-1 (MIR124-1 Human)
   

   Use

   Lentiviral

   Tags

   GFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FXN gRNA (BRDN0001146360)

   Plasmid

   #77815

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FXN

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FXN (FXN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_016-sgATF4-1

   Plasmid

   #202455

   Purpose

   Expression of a guide RNA targeting ATF4

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   ATF4 gRNA (ATF4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCENPTΔC-dCas9-3xGFP

   Plasmid

   #198325

   Purpose

   Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   CENPTΔC (CENPT Human)
   

   Use

   Lentiviral

   Tags

   EGFP x 3 and dCas9

   Expression

   Mammalian

   Mutation

   truncation - encodes only amino acids 1-375

   Promoter

   CMV-TetO

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. DGKQ gRNA (BRDN0001162275)

   Plasmid

   #77377

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DGKQ

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DGKQ (DGKQ Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SRC gRNA (BRDN0001145402)

   Plasmid

   #75650

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SRC

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SRC (SRC Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB09-TRE-beta-globin-miR-124-EF1a-GFP

   Plasmid

   #117318

   Purpose

   Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   hsa-miR-124-1 (MIR124-1 Human)
   

   Use

   Transposon-mediated integration

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-inducible promoter

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. JAK1 gRNA (BRDN0001145887)

   Plasmid

   #76392

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human JAK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   JAK1 (JAK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CDK9 gRNA (BRDN0001162576)

    Plasmid

    #77170

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK9

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK9 (CDK9 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-IRES-BLAST flag-CAD S1859A

    Plasmid

    #169821

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S1859A; TCCC -> AGTC silent mutations at nt527…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. EPHA3 gRNA (BRDN0001147140)

    Plasmid

    #76745

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EPHA3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EIF2AK4 gRNA (BRDN0001146469)

    Plasmid

    #75876

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-U6-sgMettl3

    Plasmid

    #196263

    Purpose

    Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

    Depositor

    Laura Attardi

    Article

    Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

    Insert

    Mettl3 sgRNA (Mettl3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MAP2K2 gRNA (BRDN0001147931)

    Plasmid

    #77603

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP2K2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAPK3 gRNA (BRDN0001147876)

    Plasmid

    #75967

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

    Plasmid

    #100716

    Purpose

    B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting ATP1A1 (cloned using BsmBI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1.shCDK8

    Plasmid

    #19760

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    small hairpin RNA against CDK8 (CDK8 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

19. CDK15 gRNA (BRDN0001146647)

    Plasmid

    #76485

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK15

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK15 (CDK15 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CDK15 gRNA (BRDN0001144734)

    Plasmid

    #76486

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK15

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK15 (CDK15 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. NEK1 gRNA (BRDN0001145167)

    Plasmid

    #76128

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NEK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NEK1 (NEK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CA-RIT-NFAT1 (PGK-Puro)

    Plasmid

    #63214

    Purpose

    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection marker

    Depositor

    Anjana Rao

    Article

    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

    Insert

    CA-RIT-NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

23. ERN1 gRNA (BRDN0001146341)

    Plasmid

    #76408

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ERN1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ERN1 (ERN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ERN1 gRNA (BRDN0001162304)

    Plasmid

    #76407

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ERN1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ERN1 (ERN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MAP2K2 gRNA (BRDN0001147805)

    Plasmid

    #77600

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP2K2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CDK13 gRNA (BRDN0001148050)

    Plasmid

    #76626

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK13

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK13 (CDK13 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXPR_sgFLNB-SK2

    Plasmid

    #119873

    Purpose

    gRNA expression vector

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    sgFLNB-SK2 (FLNB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

    Plasmid

    #161923

    Purpose

    To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    sgRNA targeting HSD17B11 exon 1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BPK1520-LRRK2-G2019S-ng

    Plasmid

    #180436

    Purpose

    Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MAPK3 gRNA (BRDN0001149014)

    Plasmid

    #75966

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge