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pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-ETV6-ETS

Plasmid

#111180

Purpose

Minimal ETS domain of human ETV6 (TEL)

Depositor

Gregory Poon

Article

Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

Insert

ETV6 ETS domain (ETV6 Bovine, Human, Mouse)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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p6346 MSCV-CMV-Flag-HA-Brd4-1-444

Plasmid

#32886

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 (BRD4 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAV10.KN

Plasmid

#63209

Purpose

pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PIK3R1-3'UTR

Plasmid

#182252

Purpose

Human PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PIK3R1 gene

Use

Luciferase

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB1alpha2_SulR

Plasmid

#186425

Purpose

transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Insert

SulR

Use

Synthetic Biology; Binary vector for escherichia …

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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DHFR-GFP-th-GluA1

Plasmid

#173008

Purpose

For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-GFP-th-GluA1

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMS18: pHelper(PmcCAST)_PmcPSP1

Plasmid

#168151

Purpose

Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-ErbB4CTF

Plasmid

#17803

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

HA

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pIN4_tsp-6_mScarlet

Plasmid

#182935

Purpose

Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Promoter

tsp-6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGex-6p2_FANCM

Plasmid

#221379

Purpose

Expresses FANCM in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Fanconi anemia group M protein (FANCM Human)

Expression

Bacterial

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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TAAR9-Tango

Plasmid

#66513

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

TAAR9 (TAAR9 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pB80-FKBP-mCherry-RAB11

Plasmid

#174622

Purpose

Rapalog-inducible coupling to recycling endosomes (RAB11) via FKBP

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

FKBP-mCherry-RAB11 (RAB11A Human, Synthetic)

Tags

FKBP-mCherry

Expression

Mammalian

Mutation

mCherry: Met1Del; RAB11: Met1Del

Promoter

Chicken beta-actin

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47454

Purpose

epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-SID4X

Use

AAV

Tags

2A_phiLOV2.1

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAG665 MAP4-pFAST

Plasmid

#172865

Purpose

Expresses MAP4-pFAST in mammalian cells (microtubule labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

MAP4-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159862

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GST-GAR

Plasmid

#34697

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

Insert

Fibrillarin (FBL Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-145 of human fibrillarin with K2E and …

Promoter

tac

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
promoter J23110 (upstream of RBS, mRFP1)

Plasmid

#117852

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

medium-high promoter, RBS, mRFP1

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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