We narrowed to 17,661 results for: URE

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pCAD16_CAD_12-490_pvp008

Plasmid

#124842

Purpose

Expresses Aspergillus terreus CAD in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-11, synthetic codon optimiz…

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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AM77_pU6.Sa-gRNA.CLYBL

Plasmid

#199237

Purpose

Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

S. aureus gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ5 BRG1 DN

Plasmid

#17874

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg DN (SMARCA4 Human)

Expression

Mammalian

Mutation

dominant negative

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

Plasmid

#168153

Purpose

Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B

Plasmid

#136012

Purpose

EIF3B 3'UTR (NM_001037283.1) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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bArr2-ALFA

Plasmid

#201498

Purpose

Expression of the beta-arrestin2 containing a N-terminal ALFA tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr2-ALFA (ARRB2 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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TT MS-Sensor

Plasmid

#199789

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

TT MS-Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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U6-sgRNAs-Crym

Plasmid

#200067

Purpose

For the knock down of Crym with an astrocyte specific mCherry expression

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

U6-3xsgRNA-Crym

Use

AAV, CRISPR, and Mouse Targeting

Tags

GfaABC1D-mCherry

Promoter

U6, GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-C–SaKKH-Cas9(D10A)

Plasmid

#157840

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Use

CRISPR

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-p97-E578Q

Plasmid

#85672

Purpose

Expression of human p97 E578Q mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

p97 (Vcp Rat)

Tags

GFP

Expression

Mammalian

Mutation

E578Q

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-KKHSaCas9-BlastR

Plasmid

#107189

Purpose

Confers constitutive expression of KKH SaCas9.

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Insert

KKH SaCas9 (NEWENTRY )

Expression

Bacterial

Mutation

E782K/N968K/R1015H

Promoter

Chicken ß-Actin

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.shHmga2

Plasmid

#32399

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

shRNA targetting Hmga2 (Hmga2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MYC dHLH

Plasmid

#176047

Purpose

Expresses Myc Mutant (delta helix-hoop-helix) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Expression

Mammalian

Mutation

deleted amino acids 371-412

Promoter

CMV

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

Plasmid

#179586

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NeonGreen

Promoter

TRE

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-Noxa-pEGFP-C1

Plasmid

#166738

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Noxa (PMAIP1 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMJA103

Plasmid

#128519

Purpose

Expresses human amyloid Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme D67H (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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AsCas12a-2C-NLS in pCSDest

Plasmid

#126637

Purpose

Expresses AsCas12a-2C-NLS in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO ddd64.1

Plasmid

#123121

Purpose

E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Mutation

Mutated amino acids in catalytic triad to alanine…

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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HS-PCNA-GFP

Plasmid

#105942

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

PCNA (PCNA Human)

Expression

Mammalian

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS5

Plasmid

#218714

Purpose

Expresses N-terminally EGFP-tagged BBS5 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS5 (BBS5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pIND-hygro-HA-H3.3

Plasmid

#47981

Purpose

Ponasterone A-inducible expression of human HA tagged H3F3A

Depositor

Bing Zhu

Article

Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

Insert

H3F3A (H3-3A Human)

Tags

HA

Expression

Mammalian

Mutation

288 bp T to C synonymous mutation

Promoter

Minimal Heat Shock Promoter

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
CYFIP2.mCherry

Plasmid

#122052

Purpose

Expresses human CYFIP2 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP2 (CYFIP2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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SPLINTR GFP v1 backbone

Plasmid

#179777

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-GLIS1 (FLJ33315SAAN)

Plasmid

#33274

Purpose

Retroviral expression of canonical human GLIS1

Depositor

Shinya Yamanaka

Article

Maekawa et al Nature. 2011 . 474(7350):225-9.

Insert

GLIS1, Gateway casette A (GLIS1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pSP64T-hLbCpf1

Plasmid

#107187

Purpose

plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Promoter

sp6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OTmut

Plasmid

#185389

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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AMPK-β2-MYC

Plasmid

#40606

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pBABE-2XFLAG,2XHA-CyclinF-puro

Plasmid

#32976

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pCEP4-HA-Flag-natT1R2

Plasmid

#113945

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Promoter

CMV

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

Plasmid

#107319

Purpose

Expresses SpCas9 fused to dSaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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