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Showing: 6881 - 6900 of 11595 results

  2. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Article
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Synthetic, Photinus pyralis, Human)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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  3. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217767
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400
    Promoter
    T7 promoter
    Available Since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217767-plasmid-map-sequence-id-435788

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  9. pelB-MBP-P13-ss

    Plasmid
    #137065
    Purpose
    E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature P13: lacks the P13 signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137065-plasmid-map-sequence-id-291955

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  17. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid
    #58414
    Purpose
    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
    Depositor
    Howard Hang, Jacob Yount
    Article
    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    HA internal
    Expression
    Mammalian
    Mutation
    Cysteines 71, 72, and 105 to Alanine; The sequenc…
    Promoter
    CMV IE
    Available Since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 6881 - 6900 of 11595 results