We narrowed to 15,838 results for: grna

1. pSIH1-puro-STAT3 shRNA

   Plasmid

   #26596

   Depositor

   Frank Sinicrope

   Article

   Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

   Insert

   STAT3 shRNA (STAT3 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUDP293

   Plasmid

   #204228

   Purpose

   Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

   Depositor

   Jean-Marc Daran

   Article

   Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

   Inserts

   Eubacterium rectale cas12a (Ercas12a)
   BsaI_GFP drop-out_BsaI
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   codon optimized for S. cerevisiae

   Promoter

   Saccharomyces cerevisiae PGK1 and Saccharomyces c…

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-tevopreq1-GG-acceptor

   Plasmid

   #174038

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-AAVS1

   Plasmid

   #227272

   Purpose

   Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting AAVS1 (AAVS1 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 Puro shRNA Scramble

   Plasmid

   #162011

   Purpose

   Lentiviral negative control vector containing scramble shRNA

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   scramble shRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBA620

   Plasmid

   #186240

   Purpose

   LbuCas13a Negative Control (RFP targeting)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   pTet-eLbuCas13a + crRNA negative control
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBA630

   Plasmid

   #186241

   Purpose

   RfxCas13d Negative Control (RFP targeting)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   pTet-RfxCas13d + crRNA negative control
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBA615

   Plasmid

   #186239

   Purpose

   eLbuCas13a Negative Control (RFP targeting)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   pTet-LbuCas13a + crRNA negative control
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-EGxxFP

    Plasmid

    #50716

    Purpose

    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    split EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    Eric Lander, David Sabatini

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCas9-sgAAVS1-1

    Plasmid

    #129726

    Purpose

    Expressing SpCas9 and sgAAVS1-1

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-1
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEE082 FoMV

    Plasmid

    #234811

    Purpose

    To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-tmpknot-GG-acceptor

    Plasmid

    #174039

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSGAb-km

    Plasmid

    #121999

    Purpose

    A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Insert

    none
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AIO-GFP

    Plasmid

    #74119

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFUChW Parkin shRNA Mul1 shRNA

    Plasmid

    #78523

    Purpose

    Lentiviral vector encoding two shRNAs and mCherry reporter

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    Park2 shRNA, Mul1 shRNA (Prkn, Mul1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. RAB14 shRNA

    Plasmid

    #61496

    Purpose

    to knockdown endogenous RAB14 expression levels

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 (RAB14 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSpyC-GFP

    Plasmid

    #220191

    Purpose

    sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRetrosuper USP28 shRNA-1

    Plasmid

    #15663

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    USP28 shRNA (USP28 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pC0041-RanCas13b crRNA backbone

    Plasmid

    #103852

    Purpose

    For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-RanCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSGAb-spe

    Plasmid

    #122000

    Purpose

    A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Insert

    none
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PLKO.1-UPF1-CDS

    Plasmid

    #136037

    Purpose

    UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRH2521

    Plasmid

    #84380

    Purpose

    Expression of sgRNA from a imyc promoter (Pimyc)

    Depositor

    Robert Husson

    Article

    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

    Insert

    sgRNA cloning site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO-PTEN-shRNA-1320

    Plasmid

    #25638

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-1320 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO-PTEN-shRNA-3001

    Plasmid

    #25639

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-3001 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ131C

    Plasmid

    #69284

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge