19,660 results

1. pCRISPRi_Mxi1_yl

   Plasmid

   #91248

   Purpose

   CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-N2cG

   Plasmid

   #100811

   Purpose

   express rabies virus CVS N2c strain glycoprotein gene (N2cG)

   Depositor

   Ian Wickersham

   Insert

   rabies virus CVS N2c strain glycoprotein gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWB533

   Plasmid

   #74872

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPkan-S

   Plasmid

   #41017

   Purpose

   PCR template for en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   Kana_I-SceI
   

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Puro-Cas9 donor

   Plasmid

   #58409

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. spCas9-BlastR (pCBhCas9-BlastR)

   Plasmid

   #71489

   Purpose

   Expresses SpCas9 and Blasticidin resistance

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Insert

   spCas9
   

   Promoter

   CBh

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Luc.Cre empty vector

   Plasmid

   #20905

   Depositor

   Tyler Jacks

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-RLuc8.6-535

   Plasmid

   #87125

   Purpose

   Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

   Depositor

   Sanjiv Sam Gambhir

   Article

   Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

   Insert

   Renilla Luciferase
   

   Expression

   Mammalian

   Mutation

   RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. TGFB1-bio-His

   Plasmid

   #52185

   Purpose

   Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TGFB1 (TGFB1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAF112

    Plasmid

    #135920

    Purpose

    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Type

    Empty backbone

    Use

    Luciferase; Transposon

    Expression

    Mammalian

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CWB-yellow pre-eGRASP(p32)

    Plasmid

    #111580

    Purpose

    An AAV vector that expresses yellow pre-eGRASP(p32) under the CaMKIIa promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    yellow pre-eGRASP(p32)
    

    Use

    AAV

    Promoter

    CaMKIIa

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-calpainsensor

    Plasmid

    #36182

    Purpose

    detection of activity of ubiquitous calpains

    Depositor

    Isabelle Richard

    Article

    Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

    Insert

    alpha-Fodrin cleavage site (Sptan1 Mouse)
    

    Tags

    eCFP and eYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AbVec2.0-mIghg1

    Plasmid

    #127158

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg1 (Ighg1 Mouse)
    

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CHOP promoter (-649/+136) pmCherry-1

    Plasmid

    #36035

    Depositor

    Quan Lu

    Article

    Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

    Insert

    CHOP promoter (-647 to +136) (DDIT3 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+) mCherry

    Plasmid

    #176301

    Purpose

    Expresses mCherry mRNA from CMV promoter

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p58sheLL

    Plasmid

    #37324

    Depositor

    John Schiller

    Article

    Kondo et al Virology. 2007 Feb 20;358(2):266-72. Epu

    Insert

    HPV58L1+L2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPP441

    Plasmid

    #158801

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Tags

    2xSV40NLS-2xFLAG

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6d:sgRNA#5

    Plasmid

    #64249

    Purpose

    expresses sgRNA under U6d promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6/V5-p53_R249S

    Plasmid

    #22935

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R249S Mutant

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. GPRC5A-Tango

    Plasmid

    #66382

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPRC5A (GPRC5A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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