165,271 results

1. ALPK1 gRNA (BRDN0001146035)

   Plasmid

   #76081

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ALPK1 gRNA (BRDN0001145294)

   Plasmid

   #76083

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ALPK1 gRNA (BRDN0001146875)

   Plasmid

   #76084

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ALPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. spNW30

   Plasmid

   #173485

   Purpose

   Expresses spNW30 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW30
   

   Tags

   Histag

   Expression

   Bacterial

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBT2-4xUAS:NICD-P2A-mRFP

   Plasmid

   #127594

   Purpose

   Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Insert

   NICD (notch1a Zebrafish)
   

   Use

   Zebrafish expression

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FCGR2a-bio-His

   Plasmid

   #51754

   Purpose

   Expresses full-length Low affinity immunoglobulin gamma Fc region receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   FCGR2a (FCGR2A Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSET FLIPPi-30m

   Plasmid

   #13556

   Depositor

   Wolf Frommer

   Article

   Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

   Insert

   FLIPPi T22A
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   T22A in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLJM1 polylinker

   Plasmid

   #164143

   Purpose

   Lentiviral expression vector pLJM1 with extended polylinker

   Depositor

   Richard Possemato

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-SARS-CoV-2-S (Wuhan)

   Plasmid

   #215812

   Purpose

   Expresses SARS-CoV-2 Spike protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Spike (S )
   

   Expression

   Mammalian

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-cMSP26

    Plasmid

    #199769

    Purpose

    Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP26
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-cMSPdH4-6

    Plasmid

    #199766

    Purpose

    Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH46
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX4T-1-6AlanineHE1

    Plasmid

    #165098

    Purpose

    bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1

    Depositor

    Arthur Haas

    Insert

    Uba1 (UBA1 Human)
    

    Tags

    GST-6-alanine

    Expression

    Bacterial

    Mutation

    None

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBR_attB(bxb)_lox

    Plasmid

    #183762

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

    Plasmid

    #205152

    Purpose

    Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    FRT-GFP-FRT-uORF-dCBEevoCDA1
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAST

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSCV-miR-34b/34c

    Plasmid

    #63933

    Purpose

    MSCV vector for overexpressing mouse miR-34b/34c

    Depositor

    Lin He

    Article

    Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

    Insert

    microRNA 34b/34c (Mir34c, Mir34b Mouse)
    

    Expression

    Mammalian

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pNL [NlucP/p53-RE/Hygro] Vector

    Plasmid

    #236920

    Purpose

    Express p53 response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    p53 (TP53 Human)
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAAV-mTH-GFP

    Plasmid

    #99128

    Purpose

    An AAV genome that expresses GFP from the mouse TH promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mTH

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOsTIR1w/oGFP

    Plasmid

    #102883

    Purpose

    Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    OsTIR1
    

    Tags

    3x myc

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-N-WASP

    Plasmid

    #47406

    Depositor

    Peter McPherson

    Article

    Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

    Insert

    N-WASP
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pICH54011

    Plasmid

    #48065

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pWZL hygro 12S E1A

    Plasmid

    #18748

    Depositor

    Scott Lowe

    Article

    Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

    Insert

    12S E1A
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EGFP-Rab7A

    Plasmid

    #28047

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Available Since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTXB3-Flag PLK1 T210D

    Plasmid

    #68272

    Purpose

    bacterial expression of Plk1 T210D mutant

    Depositor

    Stefano Ferrari

    Insert

    PLK1 (PLK1 Human)
    

    Tags

    Flag and Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    T210D

    Promoter

    T7

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pAT-WT-HA-GFP11

    Plasmid

    #177719

    Purpose

    ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3-STAT3-Y705F

    Plasmid

    #74434

    Purpose

    Expression of STAT3-Y705F

    Depositor

    Jie Chen

    Article

    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

    Insert

    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Mutations E16K, Y705F

    Promoter

    CMV

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid

    #166693

    Purpose

    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    TRE and U6

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EBFP2-N1

    Plasmid

    #54595

    Purpose

    Localization: N1 Cloning Vector, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    EBFP2

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pQhsCDtRNA-

    Plasmid

    #86015

    Purpose

    Multi-gene expression system with human box C/D proteins with U14

    Depositor

    Hong Li

    Article

    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

    Inserts

    U14 (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    

    Use

    Co-expression; purification

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    amino acids 1-401, amino acids 1-411, and amino a…

    Available Since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET His6 MBP TEV LIC cloning vector (2M-T)

    Plasmid

    #29708

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EBFP2-UtrCH

    Plasmid

    #124656

    Purpose

    F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    Utrophin (UTRN Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge