We narrowed to 17,810 results for: SHE

1. pDEST-FRT-TO-GFP-PALB2 KR

   Plasmid

   #71107

   Purpose

   mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   PALB2 (PALB2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   first 8 Lysine residues mutated to Arginine (K14-…

   Promoter

   CMV/TO

   Available Since

   Dec. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMV-HIS3

   Plasmid

   #65333

   Purpose

   standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   HIS3
   

   Use

   Synthetic Biology

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-ZZ-TEV-SNAPf-BICD2N(1-400)

   Plasmid

   #111857

   Purpose

   N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

   Depositor

   Andrew Carter

   Article

   Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

   Insert

   BICD2N(1-400)
   

   Tags

   His-tag, SNAPf-tag, TEV site, and ZZ

   Expression

   Insect

   Mutation

   Optimised for Sf9 expression

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 CASTOR2-HA Cterm

   Plasmid

   #84489

   Purpose

   overexpression of CASTOR2 with HA tag

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR2 (GATSL2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCDH-2xMARS-nGFP-puro

   Plasmid

   #205238

   Purpose

   Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody

   Depositor

   Jeremy Baskin

   Insert

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   

   Use

   Lentiviral

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKmyc-Borg3

   Plasmid

   #141074

   Purpose

   Expresses Borg3 (Cdc42ep5) in mammalian cells

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol. 2001 Oct;3(10):861-6. doi

   Insert

   Borg3 (Cdc42ep5 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKD280.7

   Plasmid

   #173172

   Purpose

   Expresses SO_4387

   Depositor

   Jeffrey Tabor

   Article

   Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Insert

   SO_4387
   

   Expression

   Bacterial

   Promoter

   J23107

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-TAT-2-130-FLAG

   Plasmid

   #35984

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV2 130 amino acids

   Available Since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLHCX-Flag-mPKM2(K433E)

   Plasmid

   #42514

   Depositor

   Dimitrios Anastasiou

   Article

   Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

   Insert

   Pyruvate Kinase M2 Lys433->Glu
   

   Use

   Retroviral

   Tags

   Flag

   Mutation

   Changed lysine 433 to glutamate

   Available Since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

10. Halo-Tev-Akt3 WT-2X Flag

    Plasmid

    #87607

    Purpose

    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 protein

    Depositor

    Yimon Aye

    Insert

    Akt3 isoform 2 (AKT3 Human)
    

    Use

    Zebrafish (danio rerio) expression

    Tags

    2 tandem flag tags and His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Akt3 isoform 2

    Promoter

    SP6

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPQD2KN0897

    Plasmid

    #177090

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatB

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H
    

    Use

    Synthetic Biology

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C-SLC35B2

    Plasmid

    #155037

    Purpose

    Transient expression of EGFP-SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ELC-Fc

    Plasmid

    #8636

    Depositor

    Timothy Springer

    Article

    Manjunath et al J Clin Invest 2001 Sep;108(6):871-8.

    Insert

    CCL19 (CCL19 Human)
    

    Expression

    Mammalian

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

14. POT4-RFP

    Plasmid

    #65320

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLA CMV N-Flag RALB WT

    Plasmid

    #50990

    Purpose

    Expresses RALB in mammalian cells

    Depositor

    Anna Sablina

    Article

    Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

    Insert

    RALB (RALB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pTYF-sGFAP-LOx-IRES-tdTomato

    Plasmid

    #166919

    Purpose

    Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Insert

    Lactate oxidase
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    sGFAP

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GWF180

    Plasmid

    #133607

    Purpose

    CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-NLS-FRB-VP16

    Plasmid

    #210515

    Purpose

    expresses NLS-FRB-VP16 component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NLS-FRB-VP16
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-miniSOG-T2A-dTomato

    Plasmid

    #193796

    Purpose

    Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PKC-delta-IRES-hrGFP

    Plasmid

    #73414

    Purpose

    Mammalian expression of mouse PKC-delta and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKC-delta (Prkcd Mouse)
    

    Tags

    3xFLAG and IRES-hrGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pPB-CAG-Flag3-pG-mNanog-pgk-hph

    Plasmid

    #74911

    Purpose

    Mammalian expression of Flag tagged mNanog

    Depositor

    Jose Silva

    Article

    Theunissen et al Development. 2011 Nov;138(22):4853-65. d

    Insert

    Nanog (Nanog Mouse)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. OVA-miSFIT-8-G

    Plasmid

    #124675

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGL3-24p3/LCN2-282-LUC

    Plasmid

    #46865

    Purpose

    murine 24p3 minimal promoter as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Promoter

    24p3 minimal promoter

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.1-P2X2-CFP

    Plasmid

    #22401

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

25. pKD280

    Plasmid

    #125071

    Purpose

    Expression of SO_4387 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4387 (SO_4387 )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    S286L- Please see depositor comments below

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBabe_puro_DEST_Flag_SLC2A2

    Plasmid

    #45267

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

    Plasmid

    #114729

    Purpose

    3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M5, sgRNA M7, sgRNA M9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLL3.7-hSt3Cas9 (pXK-1721)

    Plasmid

    #208828

    Purpose

    CRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.

    Depositor

    Kun Xu

    Article

    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

    Type

    Empty backbone

    Use

    CRISPR; Stcas9

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. HSD17B10

    Plasmid

    #39153

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD17B10 (HSD17B10 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pMtb CblA

    Plasmid

    #138298

    Purpose

    Expression of Mtb CblA

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Mtb CblA
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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