We narrowed to 21,472 results for: SPR
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Plasmid#108864PurposeFnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processingDepositorTypeEmpty backboneUseCRISPR; Gateway compatible crrna entry cloneExpressionPlantAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAP974-1
Plasmid#99497PurposeTEV::linker::tagRFP::3XFlagDepositorInsertTEV::linker::tagRFP::3XFlag
ExpressionBacterialAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIV643
Plasmid#226182PurposeExpresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.DepositorInsertuidA marker flanked with targeting regions to KU70 gene in K.phaffii
ExpressionBacterialPromoterTEF1pAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-BSD
Plasmid#216123PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-602_NY-ESO-1_TCR_RFP
Plasmid#207483PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertRFP, NY-ESO-1_TCR
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBA1015
Plasmid#189589PurposeT7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)DepositorInsertT7gp1.7 Locus 250bp Homology Arms
UseSynthetic BiologyExpressionBacterialMutationEncodes for a full deletion of T7gp1.7Available SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LMNA_mScarlet-I_Donor
Plasmid#178092PurposeHDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.DepositorInsertLMNA-mScarlet-I HDR donor
UseCRISPRExpressionMammalianAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G8_Dual_sgRNA
Plasmid#178104PurposeCoselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.DepositorInsertATP1A1 G8 sgRNA + user-specified sgRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSABEd
Plasmid#133968PurposeExpresses ABEd CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertABEd
UseCRISPR and Synthetic BiologyAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP L138 gRNA
Plasmid#119133PurposegRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.DepositorInserteGFP L138 gRNA
UseCRISPRMutationNonePromoterU6Available SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a MS2-HP1cs 2A Hygro
Plasmid#102810PurposeLentiviral plasmid from FIRE-Cas9 system to express MS2-HP1cs fusion proteinDepositorInsertMS2-HP1cs
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuidPuro-hTP73_sgRNA-1
Plasmid#88855PurposeCRISPR KO of Trp73DepositorInsertTrp73
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pgRNA
Plasmid#215560PurposeConstitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanRDepositorInsertgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYZ960
Plasmid#187972PurposeTEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2ยต-URA3DepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget: Ptaq-RFP_PlacIq-GFP
Plasmid#192280PurposeRFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter PlaciqDepositorInsertsRFP
GFP
ExpressionBacterialPromoterPlacIq and PtaqAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2621 (pCas8_Vch)
Plasmid#200893PurposeExpresses human codon-optimized V. cholerae Cas8 from a CMV promoter.DepositorInsertVchCas8
TagsNLSExpressionMammalianAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2623 (pCas6_Vch)
Plasmid#200895PurposeExpresses human codon-optimized V. cholerae Cas6 from a CMV promoter.DepositorInsertVchCas6
TagsNLSExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
Tags6X His and sortase A sorting motifExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only