We narrowed to 50,629 results for: ang

1. PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

   Plasmid

   #124494

   Purpose

   Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Caspase-1 cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCG150_double_phiC31_attB

   Plasmid

   #169693

   Purpose

   cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

   Depositor

   Shih-Peng Chan

   Article

   Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PCS2-FlipGFP(Casp3 cleavage seq)

   Plasmid

   #124432

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq)
   

   Use

   Zebrafish (good expression)

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-AsCas12a-Plus-mCherry

   Plasmid

   #191231

   Purpose

   To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   AsCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   E174R, R951K, R955A

   Promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-SlugABE-NNG-P2A-puro

   Plasmid

   #214354

   Purpose

   Expresses SlugABE-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugABE-NNG and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pST1374-N-NLS-flag-linker-Cas9-H840A

   Plasmid

   #51129

   Purpose

   Cas9 nickase optimized for nuclear import.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Insert

   Cas9 nickase
   

   Use

   CRISPR

   Tags

   Flag and NLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SlugCas9-HF

   Plasmid

   #163796

   Purpose

   Expresses highly specific SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   SlugCas9 (R247A, N415A, T421A, R656A)

   Promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-SlugCas9-NNG-P2A-puro

   Plasmid

   #214352

   Purpose

   Expresses SlugCas9-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9-NNG and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-SlugABE-NNG

    Plasmid

    #214356

    Purpose

    Expresses SlugABE-NNG

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-SlugCBE-NNG-P2A-puro

    Plasmid

    #214355

    Purpose

    Expresses SlugCBE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCBE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172670

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    SDHB pegRNA and SDHB_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-LbCas12a-Plus-mCherry

    Plasmid

    #191230

    Purpose

    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    LbCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R, R883K, R887A

    Promoter

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172668

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    ALDOB pegRNA and ALDOB_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172669

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    RNF2 pegRNA and RNF2_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-SpCas9HF1-P2A-puro

    Plasmid

    #214357

    Purpose

    Expresses SpCas9HF1 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9HF1 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-5U6-sgRNAs-hsyn-EGFP

    Plasmid

    #112213

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    sgRNAs
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122

    Plasmid

    #223186

    Purpose

    Plasmid containing the Zfr/YAP5SA/3xMIR122 cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

    Depositor

    Yuxuan Guo

    Article

    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

    Inserts

    YAP5SA (Yap1 Mouse)
    mir122 (Mir122 Mouse)
    

    Use

    AAV

    Tags

    HA and engineered P2A

    Expression

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    TNNT2

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-ICUE4

    Plasmid

    #181846

    Purpose

    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    ICUE4 (RAPGEF3 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-RIα-ICUE4

    Plasmid

    #181848

    Purpose

    ICUE4 cAMP sensor tethered to the PKA RIα subunit.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
    

    Use

    Tags

    ECFP, PKA RIα, and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

    Plasmid

    #181853

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMXs-KMS

    Plasmid

    #188038

    Purpose

    Polycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vector

    Depositor

    Young Tang

    Article

    Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

    Insert

    KLF4, MYC, and SOX2 separated by 2A sequences (KLF4, MYC, SOX2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124428

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pST1374-N-NLS-flag-linker-Cas9-D10A

    Plasmid

    #51130

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    D10A (nickase mutant)

    Promoter

    CMV

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124431

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Zebrafish (if expression level is too high in zeb…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid

    #196063

    Purpose

    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

    Depositor

    Wenxing Yang

    Article

    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

    Inserts

    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. YW347_Ptbb2UTR-loxP-RFP-miniMOS

    Plasmid

    #196064

    Purpose

    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

    Depositor

    Wenxing Yang

    Article

    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

    Inserts

    myo2p driven tagRFP
    NeoR
    Minimal Mos1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    myo-2

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.3-SARS2-B.1.617.2

    Plasmid

    #172320

    Purpose

    expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.2 strain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

    Promoter

    CMV-F

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-PDE4D2cat-ICUE4

    Plasmid

    #181847

    Purpose

    ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
    

    Use

    Tags

    ECFP, PDE4D2(86-418), and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.3-SARS2-B.1.617.1

    Plasmid

    #172319

    Purpose

    expressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.1 strain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

    Promoter

    CMV

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVRC8400-SARS-CoV-2-RBD-AVI

    Plasmid

    #160476

    Purpose

    Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

    Insert

    SARS-CoV-2-RBD
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pVRC8400-SARS-CoV-2-NTD-AVI

    Plasmid

    #160475

    Purpose

    Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

    Insert

    SARS-CoV-2-NTD
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.3_SARS2_Mu

    Plasmid

    #177578

    Purpose

    expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike (Mu variant) (S SARS-CoV-2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.3_SARS2_Lambda

    Plasmid

    #177579

    Purpose

    expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike (Lambda variant) (S SARS-CoV-2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVRC8400-SARS-CoV-2-S2P-AVI

    Plasmid

    #160474

    Purpose

    Expresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

    Insert

    SARS-CoV-2-S2P
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pVRC8400-SARS-CoV-2-RBD-SD1-AVI

    Plasmid

    #160477

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

    Insert

    SARS-CoV-2-RBD-SD1
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDisplay-EGFP-CAAX

    Plasmid

    #208683

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDisplay-GRAB_CRFmut

    Plasmid

    #208656

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Pdpy-30-miniCAFE

    Plasmid

    #170115

    Purpose

    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    Dpy-30

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. CMV-VPR-S-L1-dCjCas9 MiniCAFE

    Plasmid

    #169910

    Purpose

    Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    CMV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDisplay-GRAB_NPY1.0

    Plasmid

    #208675

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. AAV-hSyn-mCherry-P2A-Cre-WPRE

    Plasmid

    #107312

    Purpose

    Encoding hSyn-mCherry-Cre

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Has Service

    AAV1

    Insert

    hSyn-mCherry-P2A-Cre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pDisplay-GRAB_SST1.0

    Plasmid

    #208664

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAAV-hSyn-GRAB_SST1.0

    Plasmid

    #208665

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4

    Plasmid

    #107310

    Purpose

    Encoding dCas9-10xGCN4 driven by dSV40 promoter

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    dSV40-dCas9-10xGCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pDisplay-GRAB_CRF1.0

    Plasmid

    #208655

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge