We narrowed to 8,802 results for: ef1;
-
Plasmid#113156PurposeAn AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporterDepositorInsertTwo gRNAs for rat Rosa26
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermU6 and hU6Available sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL
Plasmid#63884PurposeAn AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.DepositorInsertER-localized GCaMP3(wt)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75233PurposeCRISPR/Cas9 plasmid against human NFATc2DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PBG-WPRE-hGH
Plasmid#74287PurposepAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent mannerDepositorInsertPBG (chimeric rabies Glycoprotein)
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-PARP11-22-122-EGFP-Hygro
Plasmid#238097PurposeExpression of structurally predicted WWE domain of PARP11 with EGFP on C terminalDepositorInsertPARP11 (PARP11 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 22-112 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-PARP12-298-462-Hygro
Plasmid#238098PurposeExpression of structurally predicted WWE domain of PARP12 with EGFP on C terminalDepositorInsertPARP12 (PARP12 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 298-462 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-PARPT-334-406-Hygro
Plasmid#238099PurposeExpression of structurally predicted WWE domain of PARPT with EGFP on C terminalDepositorInsertPARPT
UseLentiviralTagsEGFPExpressionMammalianMutationaa 334-406 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-PARP14-1535-1601-Hygro
Plasmid#238100PurposeExpression of structurally predicted WWE domain of PARP14 with EGFP on C terminalDepositorInsertPARP14 (PARP14 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1535-1601 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-ZCCHV-594-684-Hygro
Plasmid#238102PurposeExpression of structurally predicted WWE domain of ZCCHV with EGFP on C terminalDepositorInsertZCCHV
UseLentiviralTagsEGFPExpressionMammalianMutationaa 594-684 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-RNF146-45-182-EGFP-Hygro
Plasmid#238091PurposeExpression of structurally predicted WWE domain of RNF146 with EGFP on C terminalDepositorInsertRNF146 (RNF146 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 45-182 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-DTX1-1-175-EGFP-Hygro
Plasmid#238093PurposeExpression of structurally predicted WWE domain of DTX1 with EGFP on C terminalDepositorInsertDTX1 (DTX1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1-175 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-DTX2-1-178-EGFP-Hygro
Plasmid#238094PurposeExpression of structurally predicted WWE domain of DTX2 with EGFP on C terminalDepositorInsertDTX2 (DTX2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1-178 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-DTX4-1-166-EGFP-Hygro
Plasmid#238095PurposeExpression of structurally predicted WWE domain of DTX4 with EGFP on C terminalDepositorInsertDTX4 (DTX4 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1-166 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-HUWE1-1503-1780_EGFP-Hygro
Plasmid#238096PurposeExpression of structurally predicted WWE domain of HUWE1 with EGFP on C terminalDepositorInsertHUWE1 (HUWE1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1-166 onlyPromoterEF1alphaAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-DTX2(8-174)-EGFP
Plasmid#196232PurposeEGFP fused to the C-terminus of tandem WWE domains within Deltex2 & a hygromycin resistance cassetteDepositorInsertDTX2(8-174)-EGFP
UseLentiviralTagsEGFP and Myc tagExpressionMammalianMutationPromoterEF1AAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-Af1521(G42E)-myc
Plasmid#196238PurposeAf1521(G42E) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassetteDepositorInsertAf1521(G42E)-myc
UseLentiviralTagsmyc tagExpressionMammalianMutationPromoterEF1AAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-RNF146(92-168)-EGFP
Plasmid#196228PurposeEGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassetteDepositorInsertRNF146(92-168)-EGFP
UseLentiviralTagsEGFP and Myc tagExpressionMammalianMutationPromoterEF1AAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-cOpn5-T2A-EGFP
Plasmid#237858PurposeThe transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoterDepositorInsertOpn5 (OPN5 Chicken)
UseAAV and Cre/LoxTagsExpressionMutationPromoterEF1αAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-cOpn5-T2A-mcherry
Plasmid#238009PurposeThe transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoterDepositorInsertOpn5 (OPN5 Chicken)
UseAAV and Cre/LoxTagsExpressionMutationPromoterEF1αAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE
Plasmid#224936PurposepAAV vector for flippase(FLP)-dependent OCaMP (orange calcium indicator) expression under the control of EF1a promoterDepositorInsertOCaMP
UseAAVTags6xHisExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 28, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKG2436_pGEEC532_pShip-EF1a-mRuby2-P2A-PuroR-bGH
Plasmid#239728PurposePlasmid expressing mRuby2 and PuroR with the EF1a promoterDepositorInsertmRuby2-P2A-PuroR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-Ace-mNeon2-WPRE
Plasmid#240056PurposeCre dependent Green fluorescent, negative response-polarity voltage indicator for mammalian expressionDepositorInsertAce-mNeon2
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
(915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop
Plasmid#195623PurposeSleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi constructDepositorInsertdCasphi
UseTagsC-Myc (NLS), HA tag, and ZIM3ExpressionMammalianMutationP749A and P753APromoterEF1a (Mlu1/EcoR1)Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWF073_LVt-EF1a-eGFP-ffLuc-PGK-BlastR
Plasmid#233447PurposeStable expression of eGFP-firefly luciferase (ffLuc)DepositorInserteGFP-firefly luciferase (ffLuc)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH
Plasmid#235266PurposeComMAND EGFP-FMRP open-loop circuitDepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseSynthetic BiologyTagsFLAGExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH
Plasmid#235267PurposeComMAND EGFP-FMRP closed-loop circuitDepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseSynthetic BiologyTagsFLAGExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7
Plasmid#231358PurposeSingle stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.DepositorInsertT7-SP6-BC(p11-14)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α-loxP-GCaMP6f-loxP-WPRE
Plasmid#233288PurposeAAV vector with EF1α promoter and Cre-Out GCaMP6f, enabling to express GCaMP6f in Cre-negative cells.DepositorInsertGCaMP6f
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3
Plasmid#221114PurposeFAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5E-EFS (EF1a core) (JDW 1319)
Plasmid#224474PurposeGateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.DepositorInsertEFS promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)
Plasmid#194232PurposeLentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB3-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)
Plasmid#194234PurposeLentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7-S72A
Plasmid#221556PurposeExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationChange serine 72 to alaninePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-E696K-EGFP
Plasmid#221554PurposeExpresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationChanged glutamate 696 to lysinePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552 EF1a DIO Chronos GFP_Vgat gRNA
Plasmid#215277PurposeExpresses Vgat gRNA in a Cre dependent Chronos GFP vectorDepositorInsertVgat gRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552 EF1a DIO Chronos GFP_VgatTTT gRNA
Plasmid#215278PurposeExpresses Vgat Control gRNA in a Cre dependent Chronos GFP vectorDepositorInsertVgat control gRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
Plasmid#207024PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384A Y387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP
Plasmid#207023PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371A I375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
p931-SWITCH-OVER insert: EF1a-Blasti
Plasmid#217890Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP
Plasmid#216476Purposelentiviral dual expression of dsRed and tagBFPDepositorInsertsdsRed
tagBFP
UseLentiviralTagsExpressionMutationPromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep
Plasmid#213542PurposeAAV viral vector packaging plasmid that expresses CoV2DepositorInsertCoV2 Orf3a-2xStrep (ORF3a Severe acute respiratory syndrome coronavirus 2, Human)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1993 - pAAV EF1a CoV2 protE-2xStrep
Plasmid#213543PurposeAAV viral vector packaging plasmid that expresses CoV2DepositorInsertCoV2 protE-2xStrep (E Severe acute respiratory syndrome coronavirus 2, Human)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-10_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211670PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-10 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-5_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211665PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-5 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-6_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211666PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-6 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-7_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211667PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-7 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgRPA1-2_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211659PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgRPA1-2 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-4_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211664PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-4 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-21_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211671PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-21 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-22_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211672PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-22 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only