159,530 results

1. pMMTV neu NT

   Plasmid

   #1823

   Depositor

   Philip Leder

   Article

   Muller et al Cell 1988 Jul 1;54(1):105-15.

   Insert

   neu NT (Erbb2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-N2cL

   Plasmid

   #100812

   Purpose

   express rabies virus CVS N2c strain polymerase gene (N2cL)

   Depositor

   Ian Wickersham

   Insert

   rabies virus CVS N2c strain polymerase gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3_Csx29

   Plasmid

   #197606

   Purpose

   Expresses Csx29 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   Csx29
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-N2cN

   Plasmid

   #100801

   Purpose

   express rabies virus CVS N2c strain nucleoprotein gene (N2cN)

   Depositor

   Ian Wickersham

   Insert

   rabies virus CVS N2c strain nucleoprotein gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-N2cP

   Plasmid

   #100808

   Purpose

   express rabies virus CVS N2c strain phosphoprotein gene (N2cP)

   Depositor

   Ian Wickersham

   Insert

   rabies virus CVS N2c strain phosphoprotein gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYS16

   Plasmid

   #202082

   Purpose

   Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   CT mNG-T2A-PuroR Minicircle Parent
   

   Use

   CRISPR

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   None

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Myosin-IIA-GFP

   Plasmid

   #38297

   Depositor

   Matthew Krummel

   Article

   Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

   Insert

   Myosin II A (Myh9 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBiFC-PBZ-4A-VC

   Plasmid

   #110649

   Purpose

   expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Use

   Tags

   HA and VC

   Expression

   Mammalian

   Mutation

   C379A, C385A, C421A and C427A

   Promoter

   CMV

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nme2Cas9_AAV

   Plasmid

   #119924

   Purpose

   All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   AAV

   Tags

   NLS-3xHA-NLS and NLS-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tom20-CR

    Plasmid

    #171461

    Purpose

    Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDZ2FE_Pogz_1-1410

    Plasmid

    #105674

    Purpose

    Expresses Flag-tagged full-length human POGZ protein

    Depositor

    Thanos Halazonetis

    Insert

    POGZ (POGZ Human)
    

    Use

    Tags

    2X Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEXP(V5_H2B_APEX2)

    Plasmid

    #107597

    Purpose

    Expresses V5_H2B_APEX2 in mammallian cells

    Depositor

    Markus Hafner

    Article

    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

    Insert

    H2B-APEX2
    

    Use

    Tags

    H2B and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Dox inducible

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. THBS1-bio-His

    Plasmid

    #53417

    Purpose

    Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    THBS1 (THBS1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

    Plasmid

    #130995

    Purpose

    AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet-Kv2.1

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT119b

    Plasmid

    #50551

    Purpose

    Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Inserts

    CcaS
    CcaR
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1_hTRIM32

    Plasmid

    #69541

    Purpose

    Expresses human TRIM32 fused to c-terminal EGFP

    Depositor

    Martin Dorf

    Article

    Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

    Insert

    tripartite motif containing 32 (TRIM32 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB80-hKif5b(1-560)rigor-2xmNeongreen

    Plasmid

    #174649

    Purpose

    Live-cell marker for stable microtubules

    Depositor

    Lukas Kapitein

    Article

    Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

    Insert

    hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
    

    Use

    Tags

    mNeongreen-mNeongreen

    Expression

    Mammalian

    Mutation

    rigor mutation G234A

    Promoter

    Chicken beta-actin

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid

    #29663

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, GFP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMM553

    Plasmid

    #68543

    Purpose

    NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR

    Depositor

    Christopher Voigt, Timothy Lu

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge