We narrowed to 8,840 results for: FIE

Showing: 7001 - 7020 of 8840 results

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pBluescriptSKII+ U6-sgRNA(F+E) ACTB

Plasmid

#74705

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA

Use

CRISPR

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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Cherry-ICP0FxE-C3

Plasmid

#134562

Purpose

Expresses Cherry-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Tags

Cherry

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hTXLNA-3xFLAG

Plasmid

#165471

Purpose

Human TXLNA mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

taxilin alpha (TXLNA Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-memRFP

Plasmid

#224564

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

mRFP1

Use

Unspecified

Tags

Membrane localization signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB:Psmyc double green

Plasmid

#169412

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mWasabi
mWasabi CO

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTorPE-jREX-GECO1

Plasmid

#113941

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jREX-GECO1

Tags

6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMTTH+bromo5

Plasmid

#157803

Purpose

Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

bromo5

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSpyB-GFP

Plasmid

#220190

Purpose

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-miR-211

Plasmid

#29784

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

miR-211

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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pSW610 - GR-LhG4_BD

Plasmid

#115992

Purpose

entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

GR-LhG4_BD

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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JH02

Plasmid

#73511

Purpose

mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mCherryo::PH::tbb-2

Tags

mCherry

Promoter

pmex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSW454 - pUFO

Plasmid

#115978

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pUFO

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-NfsB.zf1

Plasmid

#61387

Purpose

mRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

nfsB.zf1

Use

Zebrafish expression

Mutation

E28G

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-v5.9

Plasmid

#155259

Purpose

Plasmid for the aTc-inducible expression of the v5.9

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

v5.9

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-LAMP1-EGFP

Plasmid

#169003

Purpose

Express rat LAMP1-EGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

Insert

rat LAMP1-EGFP

Use

Unspecified

Tags

EGFP

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pXGFPC-5 Pxyl-dcas9 (Sth3)

Plasmid

#133317

Purpose

expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus thermophilus #3)

Use

Unspecified

Promoter

xylose

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-shSCR

Plasmid

#29780

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pEThT-SAHS1

Plasmid

#90025

Purpose

To express His-tagged SAHS1 in bacteria

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

SAHS1

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1

Plasmid

#32466

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

Expression

Bacterial

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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