We narrowed to 22,696 results for: Sis;

1. pEG302 22aa SunTag VP64 gRNA4 (FWA)

   Plasmid

   #120249

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-TRAIL-R1

   Plasmid

   #61382

   Purpose

   Expresses HA tagged TRAIL receptor 1

   Depositor

   Francis Chan

   Article

   Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

   Insert

   TRAIL receptor 1
   

   Use

   Tags

   HA and TNF receptor leader peptide

   Expression

   Mammalian

   Mutation

   TRAIL-R1 leader peptide (aa1-23) replaced with TN…

   Promoter

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

3. UAS-u(M)Cas9

   Plasmid

   #127384

   Purpose

   Expresses Cas9 at medium level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(M)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1neo-14-3-3-CLuc

   Plasmid

   #107611

   Purpose

   measure LATS activity by Luciferase Assay

   Depositor

   Xiaolong Yang

   Article

   Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

   Insert

   pCDNA3.1hygro-BamHI-CLuc1433-NotI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV Ef1a DIO GeNL_SS hGtACR1

   Plasmid

   #233511

   Purpose

   Luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

   Inserts

   mNeonGreen
   ISN-IE
   hGtACR1
   

   Use

   AAV

   Tags

   Gaussia luciferase signal sequence

   Expression

   Mutation

   Promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. R26-H2B-mCherry HR donor vector

   Plasmid

   #137928

   Purpose

   R0SA26 knock-in vector with homologous recombination-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-H2B-mCherry-bpA with homology arms
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 10XSTAT92E–luciferase

   Plasmid

   #37393

   Depositor

   Norbert Perrimon

   Article

   Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

   Insert

   SOCS36E enhancer fragment (Socs36E Fly)
   

   Use

   Luciferase; Jak/stat reporter construct

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Hsp70

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB543

   Plasmid

   #74877

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lyn-mCherry-GAI(1-92)

   Plasmid

   #37315

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Use

   Tags

   Lyn and mCherry

   Expression

   Mammalian

   Mutation

   contains aa 1-92

   Promoter

   CMV

   Available Since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pClgn1.1-Trim41-PA-1D4

    Plasmid

    #186339

    Purpose

    Express Trim41-PA-1D4 under the mouse Clgn promoter.

    Depositor

    Masahito Ikawa

    Article

    Oura et al PLoS Genet. 2022 Jun 1;18(6):e1010241. d

    Insert

    Trim41 (Trim41 Mouse)
    

    Use

    Tags

    1D4 and PA

    Expression

    Mammalian

    Mutation

    Promoter

    pClgn1.1

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-FLEx-TetOn3G-3xHA-FLEx

    Plasmid

    #133729

    Purpose

    Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    TetOn3G-3xHA (floxed & inverted)
    

    Use

    AAV

    Tags

    3xHA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CFP-PBD

    Plasmid

    #11394

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    p21-activated kinase 1 (PAK1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pHis-hMTMR1

    Plasmid

    #100723

    Purpose

    Expression of human MTMR1 PH-PTP domain

    Depositor

    Byung Il Lee

    Article

    Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

    Insert

    MTMR1 (MTMR1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 95-607

    Promoter

    T7

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-yomRuby3-SpHIS5

    Plasmid

    #204287

    Purpose

    For C-terminal tagging using yeast optimized mRuby3

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pFA6-yomRuby3-SpHIS5
    

    Use

    Tags

    yomRuby3

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXS-IRES-Blast coUGP2

    Plasmid

    #211531

    Purpose

    Expresses codon optimized UGP2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    UGP2 (UGP2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6P-1-HsRCK_E

    Plasmid

    #146210

    Purpose

    Bacterial Expression of HsRck

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    HsRck (DDX6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWPI-E2F3

    Plasmid

    #114298

    Purpose

    Constitutive expression of the E2F3 protein

    Depositor

    Patrick Salmon

    Article

    Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

    Insert

    E2F3 (E2F3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N2-RIPK3

    Plasmid

    #78822

    Purpose

    To detect localization of RIPK3 protein in live mammalian cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pGWB412

    Plasmid

    #74806

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Dual opposing T7 promoters reporter plasmid

    Plasmid

    #156457

    Purpose

    A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Insert

    NeoR/KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. MSCV eIF4E IRES GFP

    Plasmid

    #18761

    Depositor

    Scott Lowe

    Article

    Wendel et al Nature. 2004 Mar 18. 428(6980):332-7.

    Insert

    eIF4E (Eif4e Mouse)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pUB-Cas9

    Plasmid

    #86556

    Purpose

    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Cas9 is codon optimized for C. reinhardtii

    Promoter

    UBIQUITIN10

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMRX-IPU-mTagBFP2-Sec61B

    Plasmid

    #228136

    Purpose

    Expresses mTagBFP2-Sec61B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Sec61B (SEC61B Human)
    

    Use

    Retroviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. UKNP1.7.1

    Plasmid

    #97371

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 3410 pBM272 Bcl-XL

    Plasmid

    #8772

    Depositor

    Stanley Korsmeyer

    Article

    Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

    Insert

    Bcl-XL (Bcl2l1 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pCDH-EF1a-HygroR-T2A-TetOn3G

    Plasmid

    #179887

    Purpose

    Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    TetOn3G
    

    Use

    Lentiviral

    Tags

    Hygromycin resistance

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBabe-FLAG-Neuroserpin

    Plasmid

    #58261

    Purpose

    Retroviral vector for expressing Neuroserpin in mammalian cells

    Depositor

    Joan Massague

    Article

    Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

    Insert

    Neuroserpin (SERPINI1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pmiR-report-hVEGFA

    Plasmid

    #112553

    Purpose

    3'UTR reporter assay

    Depositor

    Shusheng Wang

    Article

    Zhou et al Mol Ther. 2016 Oct;24(10):1823-1835. doi

    Insert

    VEGFA (VEGFA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDBTrp-LexABD-CRY2FL

    Plasmid

    #78210

    Purpose

    Use with Addgene 40615 for blue light transcription control in yeast

    Depositor

    Chandra Tucker

    Article

    Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

    Insert

    LexABD-CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    lexA binding domain

    Expression

    Yeast

    Mutation

    contains endogenous CRY2 NLS

    Promoter

    ADH1

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pKIF1C-mCherry

    Plasmid

    #130978

    Purpose

    Expression of KIF1C-mCherry in mammalian cells.

    Depositor

    Anne Straube

    Article

    Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

    Insert

    KIF1C-mCherry (KIF1C Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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